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anti-Non-Human Primate p53 antibody for Western Blotting

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 16-25
156
105
87
67
67
65
56
54
52
51
46
46
42
40
31
30
28
25
25
23
22
21
19
17
17
13
13
13
13
13
12
11
11
10
10
9
9
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Non-Human Primate
2129
409
327
121
101
89
57
55
29
23
19
16
15
10
10
5
5
4
4
3
3
3
3
2
2
Host
Mouse
1288
954
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is conjugated to FITC
69
64
51
46
33
32
23
23
18
18
18
17
16
15
15
15
15
15
14
14
14
14
14
14
14
14
7
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1694
743
620
536
501
448
385
256
249
156
54
39
35
18
17
16
13
12
11
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN94449
$ 323.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN94448 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 16-25 Log in to see BP53-12 7

Top referenced anti-p53 antibody for Western Blotting

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Application / Reactivity Non-Human Primate Human
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
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Western Blotting (WB) 2 Antibodies
Affinity Purification (AP) 2 Antibodies
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ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
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Flow Cytometry (FACS) 428 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 16-25
(156), (105), (87), (67), (67), (65), (56), (54), (52), (51), (46), (46), (42), (40), (31), (30), (28), (25), (25), (23), (22), (21), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Non-Human Primate
(2129), (409), (327), (121), (101), (89), (57), (55), (29), (23), (19), (16), (15), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Mouse
(1288), (954), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is conjugated to FITC
(69), (64), (51), (46), (33), (32), (23), (23), (18), (18), (18), (17), (16), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1694), (743), (620), (536), (501), (448), (385), (256), (249), (156), (54), (39), (35), (18), (17), (16), (13), (12), (11), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN94449) Images
Specificity The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells, it is frequently mutated or inactivated in many types of cancer.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Immunogen Bacterially expressed full-length wild-type p53
Clone BP53-12
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN94449) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Research Area Transcription Factors, Apoptosis/Necrosis, DNA/RNA, Cell Cycle, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN94449) Images back to top
Application Notes The reagent is designed for direct immunofluorescence analysis.Note: This conjugate is routinely tested by flow cytometry analysis using permeabilized transient wild-type p53-tranfectants.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN94449) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-p53 Antibody (ABIN94449)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Tanigawa, Fujii, Hou: "Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells." in: Bioscience, biotechnology, and biochemistry, Vol. 72, Issue 3, pp. 797-804, 2008

Taylor, Agarwal, Agarwal, Stacey, Stark: "p53 inhibits entry into mitosis when DNA synthesis is blocked." in: Oncogene, Vol. 18, Issue 2, pp. 283-95, 1999

Taylor, DePrimo, Agarwal, Agarwal, Schönthal, Katula, Stark: "Mechanisms of G2 arrest in response to overexpression of p53." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3607-22, 1999

Agarwal, Agarwal, Taylor, Stark: "p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 18, pp. 8493-7, 1995

Product cited in:

Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, 1997

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, 1991

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN94449) back to top
Supplier Images
Confocal Microscopy (CM) image for anti-p53 antibody (Tumor Protein P53) (AA 16-25) (FITC) (ABIN94449) Confocal microscopy of human HeLa cells using anti-p53 (BP53-12 ; FITC). The expressi...