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anti-Rat (Rattus) p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Reactivity
Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
2037
372
306
116
101
93
55
54
27
23
19
16
13
10
10
5
5
4
4
3
3
2
2
Host
Mouse
1220
914
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
1636
720
558
487
435
421
396
234
194
156
53
38
35
17
16
12
11
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN967409
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001037418 ABIN152249 ICC FACS IHC (fro) IF IHC (p) IP IHC ELISA WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 18
0.001037418 ABIN492445 EIA FACS IHC (fro) IHC (p) IP RIA WB Mouse IgG1 AA 46-53 Log in to see Pab 1801
0.001037418 ABIN187567 FACS IF IP Mouse IgG2b Wild Type, Mutant, AA 370-378 Log in to see Pab 122
0.001037418 ABIN4342785 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.001037418 ABIN4342786 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.001037418 ABIN4342784 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.001037418 ABIN4342787 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.001037418 ABIN4342788 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.001037418 ABIN4342792 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342794 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342793 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342877 ELISA FACS ICC IF IHC (fro) IP WB Allophycocyanin Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342881 ELISA FACS ICC IF IHC (fro) IP WB DyLight 488 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342879 ELISA FACS ICC IF IHC (fro) IP WB DyLight 650 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342885 ELISA FACS ICC IF IHC (fro) IP WB PE Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342889 ELISA FACS ICC IF IHC (fro) IP WB DyLight 405LS Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342887 ELISA FACS ICC IF IHC (fro) IP WB DyLight 350 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342878 ELISA FACS ICC IF IHC (fro) IP WB Biotin Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342880 ELISA FACS ICC IF IHC (fro) IP WB DyLight 680 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.001037418 ABIN4342876 ELISA FACS ICC IF IHC (fro) IP WB Alexa Fluor 700 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122

Top referenced anti-p53 antibody for Flow Cytometry

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General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(2037), (372), (306), (116), (101), (93), (55), (54), (27), (23), (19), (16), (13), (10), (10), (5), (5), (4), (4), (3), (3), (2), (2)
Host Mouse
(1220), (914), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(1636), (720), (558), (487), (435), (421), (396), (234), (194), (156), (53), (38), (35), (17), (16), (12), (11), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN967409) Images
Brand BD Pharmingen™
Cross-Reactivity Human, Rat (Rattus), Hamster, Monkey
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4)
Clone PAb 122
Isotype IgG2b

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN967409) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers.1 Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and it forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. p53 migrates at a reduced molecular weight of 53 kDa. PAb 122 recognizes mammalian p53, including mouse, rat, hamster, monkey and human p53.2 Cells from the SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4) were used as immunogen. PAb 122 was originally evaluated by ELISA, immunoprecipitation, radioimmunoassays and indirect immunofluorescence of cultured cells.
Molecular Weight 53 kDa
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN967409) Images back to top
Application Notes Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (2 µg/ml), immunofluorescence microscopy of cultured cells and flow cytometry. COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651) or another SV40-transformed cell line are suggested as positive controls for detecting p53. COS-7 cell lysate is optimized for a western blot control and comes ready to load on an SDS-PAGE gel.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN967409) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-p53 Antibody (ABIN967409)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Legros, Lacabanne, dAgay, Larsen, Pla, Soussi: "Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells." in: Bulletin du cancer, Vol. 80, Issue 2, pp. 102-10, 1994

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991

Diller, Kassel, Nelson, Gryka, Litwak, Gebhardt, Bressac, Ozturk, Baker, Vogelstein: "p53 functions as a cell cycle control protein in osteosarcomas." in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5772-81, 1990

Yewdell, Gannon, Lane: "Monoclonal antibody analysis of p53 expression in normal and transformed cells." in: Journal of virology, Vol. 59, Issue 2, pp. 444-52, 1986

Background publications

Gurney, Harrison, Fenno: "Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens." in: Journal of virology, Vol. 34, Issue 3, pp. 752-63, 1980

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN967409) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN967409) Western blot analysis of p53. Lysate from COS-7 SV40 transformed monkey kidney cells ...
 image for anti-p53 antibody (Tumor Protein P53) (ABIN967409) anti-Tumor Protein P53 (TP53) antibody (Image 2)
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN967409) anti-Tumor Protein P53 (TP53) antibody (Image 3)