anti-Rat (Rattus) p53 antibody for Immunofluorescence

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 14-389
175
125
91
84
74
71
70
62
59
55
50
49
48
45
42
38
32
31
28
23
22
20
17
16
15
15
15
15
15
15
14
11
10
10
10
9
9
8
8
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Yeast
2387
516
423
186
127
94
85
82
35
23
20
18
17
15
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1365
1133
7
3
3
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
46
45
31
31
26
25
25
25
25
25
25
24
24
24
22
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1915
767
746
732
625
614
431
345
313
179
72
41
32
31
20
17
17
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN152249
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.030214 ABIN2957704 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
11.030214 ABIN1535528 IF IHC ELISA Rabbit IgG acLys379 Log in to see Polyclonal
11.030214 ABIN1535527 IF ELISA WB Rabbit IgG acLys317 Log in to see Polyclonal
11.030214 ABIN1532374 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
11.030214 ABIN1535547 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
11.030214 ABIN1535549 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
11.030214 ABIN2705092 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.030214 ABIN2705091 IC IF IP IHC WB Rabbit C-Term, pSer315 Log in to see Polyclonal
11.030214 ABIN2705093 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.030214 ABIN2705096 IC IF IHC WB Rabbit C-Term, acLys386 Log in to see Polyclonal
11.030214 ABIN2705097 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.030214 ABIN2707674 IC IF IP IHC WB Rabbit N-Term, pSer15 Log in to see Polyclonal
11.030214 ABIN1870674 IF WB Rabbit IgG pSer15 Log in to see Polyclonal
1 ABIN967409 FACS IF IP WB Mouse IgG2b Log in to see PAb 122 5
1 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
1 ABIN126922 IF IHC (p) IP WB Mouse IgG1 Ser20 Log in to see C6A5
1 ABIN3181875 ELISA IF IHC Rabbit IgG acLys381 Log in to see Polyclonal
1 ABIN3182104 ELISA IF IHC IP WB Rabbit IgG pSer15 Log in to see Polyclonal
1 ABIN3186283 ELISA IF IHC WB Rabbit IgG Tyr21 Log in to see Polyclonal
1 ABIN3186272 ELISA IF IHC WB Rabbit IgG Tyr191 Log in to see Polyclonal

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 14-389
(175), (125), (91), (84), (74), (71), (70), (62), (59), (55), (50), (49), (48), (45), (42), (38), (32), (31), (28), (23), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (14), (11), (10), (10), (10), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Yeast
(2387), (516), (423), (186), (127), (94), (85), (82), (35), (23), (20), (18), (17), (15), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1365), (1133), (7), (3), (3), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (54), (46), (45), (31), (31), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (22), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1915), (767), (746), (732), (625), (614), (431), (345), (313), (179), (72), (41), (32), (31), (20), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN152249) Images
Specificity This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions.
No Cross-Reactivity Xenopus laevis
Purification Protein G purified
Immunogen Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
Clone PAb 240
Isotype IgG1 kappa

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN152249) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Gene ID 7157
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN152249) Images back to top
Application Notes Western Blot 1:1000-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence 5 μg/mL, Immunoprecipitation 10 μg/mg, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen, CyTOF-readyAntigen retrieval with IHC-P is not essential but may optimise staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 1394225) Use in Immunohistochemistry-Frozen reported in various pieces of scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN152249) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-p53 antibody (ABIN152249)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Graham, Minasyan, Lomova, Cass, Balanis, Friedman, Chan, Zhao, Delgado, Go, Beck, Hurtz, Ng, Qiao, Ten Hoeve, Palaskas, Wu, Müschen, Multani, Port, Larson, Schultz, Braas, Christofk, Mellinghoff et al.: "Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. ..." in: Molecular systems biology, Vol. 13, Issue 2, pp. 914, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Shi, Ma, Zhou, Fu: "Renal Protective Effects of 17β-Estradiol on Mice with Acute Aristolochic Acid Nephropathy." in: Molecules (Basel, Switzerland), Vol. 21, Issue 10, 2016

Ge, Pettan-Brewer, Morton, Carter, Fatemi, Rabinovitch, Ladiges: "Mitochondrial catalase suppresses naturally occurring lung cancer in old mice." in: Pathobiology of aging & age related diseases, Vol. 5, pp. 28776, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Biswas, Mitchell, Johnson: "E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis." in: Cancer research, Vol. 74, Issue 12, pp. 3369-77, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lange, Bedford, Reh, Wittschieben, Carbajal, Kusewitt, DiGiovanni, Wood: "Dual role for mammalian DNA polymerase ζ in maintaining genome stability and proliferative responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 8, pp. E687-96, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Jensen, Factor, Fantozzi, Helin, Huh, Thorgeirsson: "Reduced hepatic tumor incidence in cyclin G1-deficient mice." in: Hepatology (Baltimore, Md.), Vol. 37, Issue 4, pp. 862-70, 2003 (Sample species: Mouse (Murine)). Further details: Western Blotting

Asher, Lotem, Cohen, Sachs, Shaul: "Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 3, pp. 1188-93, 2001 (Sample species: Mouse (Murine)). Further details: Western Blotting

Jones, Turner, McIlwrath, Brown, Dive: "Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53." in: Molecular pharmacology, Vol. 53, Issue 5, pp. 819-26, 1998 (Sample species: Human). Further details: Immunoprecipitation

Blagosklonny, Toretsky, Bohen, Neckers: "Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8379-83, 1996 (Sample species: Yeast). Further details: Immunoprecipitation

Bargonetti, Manfredi, Chen, Marshak, Prives: "A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein." in: Genes & development, Vol. 7, Issue 12B, pp. 2565-74, 1994 (Sample species: Human). Further details: Western Blotting

Rubio, von Deimling, Yandell, Wiestler, Gusella, Louis: "Accumulation of wild type p53 protein in human astrocytomas." in: Cancer research, Vol. 53, Issue 15, pp. 3465-7, 1993 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Halazonetis, Davis, Kandil: "Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA." in: The EMBO journal, Vol. 12, Issue 3, pp. 1021-8, 1993 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Eccles, Brett, Lessells, Gruber, Lane, Steel, Leonard: "Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma." in: British journal of cancer, Vol. 65, Issue 1, pp. 40-4, 1992 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Somers, Merrick, Lopez, Incognito, Schechter, Casey: "Frequent p53 mutations in head and neck cancer." in: Cancer research, Vol. 52, Issue 21, pp. 5997-6000, 1992 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Remvikos, Tominaga, Hammel, Laurent-Puig, Salmon, Dutrillaux, Thomas: "Increased p53 protein content of colorectal tumours correlates with poor survival." in: British journal of cancer, Vol. 66, Issue 4, pp. 758-64, 1992 (Sample species: Human). Further details: ELISA,Flow Cytometry

Cunningham, Lust, Schaid, Bren, Carpenter, Rizza, Kovach, Thibodeau: "Expression of p53 and 17p allelic loss in colorectal carcinoma." in: Cancer research, Vol. 52, Issue 7, pp. 1974-80, 1992 (Sample species: Human). Further details: Immunohistochemistry

Farrell, Allan, Shanahan, Vousden, Crook: "p53 is frequently mutated in Burkitt's lymphoma cell lines." in: The EMBO journal, Vol. 10, Issue 10, pp. 2879-87, 1991 Further details: Immunoprecipitation

Martinez, Georgoff, Martinez, Levine: "Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein." in: Genes & development, Vol. 5, Issue 2, pp. 151-9, 1991 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Davidoff, Humphrey, Iglehart, Marks: "Genetic basis for p53 overexpression in human breast cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 5006-10, 1991 (Sample species: Human). Further details: Immunohistochemistry

Gannon, Greaves, Iggo, Lane: "Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form." in: The EMBO journal, Vol. 9, Issue 5, pp. 1595-602, 1990 Further details: Western Blotting,Immunoprecipitation,ELISA,Immunohistochemistry

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN152249) back to top
Supplier Images
 image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Flow (Intracellular): p53 Antibody (PAb 240) [ABIN152249] - An intracellular stain wa...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [ABIN152249] - p53 was detected...
Immunofluorescence (IF) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [ABIN152249] - HeLa ce...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Western Blot: p53 Antibody (PAb 240) [ABIN152249] - WB analysis of p53 in MCF7 and He...