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anti-Rat (Rattus) p53 antibody for Radioimmunoassay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 46-53
123
87
62
54
50
50
49
48
46
46
43
40
39
30
27
25
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
1760
319
268
99
34
19
13
11
11
10
10
9
5
4
4
4
3
3
3
2
2
2
1
Host
Mouse
1235
612
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
59
49
34
31
23
23
15
15
14
14
14
14
14
14
13
4
4
4
4
4
4
4
4
4
4
3
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
1378
582
529
319
314
302
234
156
98
87
52
39
35
16
16
12
12
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN492445
$ 335.50
Plus shipping costs $45.00

Similar anti-p53 Antibodies

Application / Reactivity Rat (Rattus) Human
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Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 51 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 66 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 36 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 184 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 11 Antibodies
Dot Blot (DB) 31 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 46-53
(123), (87), (62), (54), (50), (50), (49), (48), (46), (46), (43), (40), (39), (30), (27), (25), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(1760), (319), (268), (99), (34), (19), (13), (11), (11), (10), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1)
Host Mouse
(1235), (612), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(59), (49), (34), (31), (23), (23), (15), (15), (14), (14), (14), (14), (14), (14), (13), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(1378), (582), (529), (319), (314), (302), (234), (156), (98), (87), (52), (39), (35), (16), (16), (12), (12), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Specificity Detects ~53 kDa.
Cellular Localization: Cytoplasmic, Endoplasmic reticulum and Nuclear.
Characteristics Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Purification Protein A Chromatography.
Immunogen Fusion protein (Human), amino acids 46-53Remarks: Mol. Wt. of Antigen: 53 kDa
Clone Pab 1801
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50 % of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes ELISA. Immunoprecipiation: Use at 10 μg/mL of Lysate. Flow Cytometry. Radioimmunoassay. Immunohistochemistry on Frozen Sections: 1/100-1/250. Immunohistochemistry on Paraffin Sections: 2-4 μg/mL for 30 min at RT, staining offormalin/paraffin tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0,for 10-20 min followed by cooling at RT for 20 min. Western blot: 1/1000-1/2000.1 μg/mL was sufficient for detection of p53 in 10 μg of Rat brain lysate by colorimetricimmunoblot analysis using Goat anti-Mouse IgG: HRP as the secondary antibody.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (AA 46-53) (ABIN492445) anti-Tumor Protein P53 (TP53) (AA 46-53) antibody