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anti-Sheep (Ovine) p53 antibody for Enzyme Immunoassay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 20-25
164
110
88
64
64
63
54
51
51
50
48
46
41
39
31
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine)
2048
381
310
124
101
93
56
54
27
23
19
16
13
11
9
9
5
4
4
3
2
2
2
1
Host
Mouse
1241
915
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1657
717
558
487
437
427
397
236
196
156
53
39
35
16
16
13
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN120054
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870064 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4

Top referenced anti-p53 antibody for Enzyme Immunoassay

Similar anti-p53 Antibodies

Application / Reactivity Sheep (Ovine) Human
Enzyme Immunoassay (EIA) 2 Antibodies 17 Antibodies
Flow Cytometry (FACS) 1 Antibodies 382 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 2 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA 665 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 191 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(164), (110), (88), (64), (64), (63), (54), (51), (51), (50), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine)
(2048), (381), (310), (124), (101), (93), (56), (54), (27), (23), (19), (16), (13), (11), (9), (9), (5), (4), (4), (3), (2), (2), (2), (1)
Host Mouse
(1241), (915), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1657), (717), (558), (487), (437), (427), (397), (236), (196), (156), (53), (39), (35), (16), (16), (13), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN120054) Images
Specificity This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
Cross-Reactivity (Details) Species reactivity (tested):Human, Horse, Bovine, Cat and Sheep.
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Clone DO-1
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN120054) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN120054) Images back to top
Application Notes Flow Cyometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/25): Heat induced epitope retrieval with 0.01 M citrate buffer, pH 6.0 is recommended. Positive Control Tissue: Colon or breast carcinoma. ELISA. Western Blotting (1/1000). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN120054) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN120054)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Sironi, Riccaboni, Mertel, Cammarata, Brooks: "p53 protein expression in conjunctival squamous cell carcinomas of domestic animals." in: Veterinary ophthalmology, Vol. 2, Issue 4, pp. 227-231, 2001

Levesque, DCosta, Angelopoulou, Diamandis: "Time-resolved immunofluorometric assay of p53 protein." in: Clinical chemistry, Vol. 41, Issue 12 Pt 1, pp. 1720-9, 1996

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992