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anti-Human PML antibody for Flow Cytometry

Recommended PML Antibody (supplied by: Log in to see )

Antigen
Promyelocytic Leukemia (PML) Antibodies
  • PML
  • MYL
  • PP8675
  • RNF71
  • TRIM19
  • 1200009E24Rik
  • AI661194
  • Trim19
  • RGD1562602
  • promyelocytic leukemia
  • PML
  • Pml
Epitope
AA 470-499, Sumoylation Site
15
14
8
7
7
6
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
117
29
11
2
2
2
1
1
1
1
Host
Rabbit
94
40
Clonality
Polyclonal
Conjugate
This PML antibody is un-conjugated
5
5
3
3
3
3
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
117
45
42
42
27
24
17
6
6
5
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN3032330
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN389073 FACS IF IHC (p) WB Rabbit Ig AA 470-499, pSumoylation Site Log in to see Polyclonal 2
1 ABIN5531863 FACS IF IHC (p) WB Rabbit Ig Fraction AA 470-499, Sumoylation Site Log in to see Polyclonal
1 ABIN1818566 ICC FACS Rabbit IgG Log in to see Polyclonal
1 ABIN1818428 ICC FACS IP WB Mouse IgG1 Log in to see 1B9
1 ABIN1840877 FACS IF IHC (p) WB Rabbit AA 470-499 Log in to see Polyclonal
1 ABIN5540563 FACS ICC IF WB Mouse IgG1 Log in to see 1B9

Top referenced anti-PML antibody for Flow Cytometry

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Immunochromatography (IC) 2 Antibodies
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Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
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General

Antigen Promyelocytic Leukemia (PML) Antibodies
Epitope AA 470-499, Sumoylation Site
(15), (14), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (29), (11), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(94), (40)
Clonality
Polyclonal
Conjugate This PML antibody is un-conjugated
(5), (5), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(117), (45), (42), (42), (27), (24), (17), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1)
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Product Details anti-PML Antibody

Target Details PML Application Details Handling Images
Purification Antigen affinity purified
Immunogen A portion of amino acids 470-499 (sumoylation site) from the human protein was used as the immunogen for this PML antibody.
Isotype Ig Fraction

Target Details PML

Product Details anti-PML Antibody Application Details Handling Images back to top
Antigen
Alternative Name PML (PML Antibody Abstract)
Background PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).
UniProt P29590
Research Area Cancer
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus

Application Details

Product Details anti-PML Antibody Target Details PML Handling Images back to top
Application Notes Titration of the PML antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-PML Antibody Target Details PML Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the PML antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-PML Antibody Target Details PML Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-PML antibody (Promyelocytic Leukemia) (AA 470-499) (ABIN3032330) Confocal immunofluorescent analysis of PML antibody with HeLa cells followed by Alexa...
Flow Cytometry (FACS) image for anti-PML antibody (Promyelocytic Leukemia) (AA 470-499) (ABIN3032330) PML antibody flow cytometric analysis of HeLa cells (right histogram) compared to a n...
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (AA 470-499) (ABIN3032330) Western blot analysis of PML antibody and HeLa lysate. There are multiple isoforms fr...