anti-Human PML antibody for Western Blotting

Recommended PML Antibody (supplied by: Log in to see )

Antigen
Promyelocytic Leukemia (PML) Antibodies
  • PML
  • MYL
  • PP8675
  • RNF71
  • TRIM19
  • 1200009E24Rik
  • AI661194
  • Trim19
  • RGD1562602
  • promyelocytic leukemia
  • PML
  • Pml
Epitope
N-Term
18
14
14
8
7
7
6
5
5
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
138
44
12
2
2
2
1
1
1
1
Host
Rabbit
113
41
1
Clonality
Polyclonal
Conjugate
This PML antibody is un-conjugated
7
6
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
135
59
58
46
43
38
17
16
7
6
5
4
3
3
1
1
1
1
Options
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Catalog No. ABIN2780446
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3471746 ABIN2778427 WB Rabbit Middle Region Log in to see Polyclonal 1
3.3471746 ABIN389073 FACS IF IHC (p) WB Rabbit Ig AA 470-499, pSumoylation Site Log in to see Polyclonal 2
3.3471746 ABIN394032 IF WB Mouse IgG2a kappa AA 411-510 Log in to see 1D12 5
3.3471746 ABIN252967 ICC IF IHC (p) IP IHC PLA WB Rabbit AA 375-425 Log in to see Polyclonal 21
3.3471746 ABIN252968 ICC IF IHC IP WB Rabbit AA 575-625 Log in to see Polyclonal 2
3.3471746 ABIN1533381 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.3471746 ABIN3186510 ELISA IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
3.3471746 ABIN452172 WB Mouse IgG1 Log in to see B3E9
3.3471746 ABIN2707709 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
3.3471746 ABIN4346485 IP WB Rabbit IgG N-Term Log in to see Polyclonal
3.3471746 ABIN2457998 ELISA WB Rabbit Log in to see Polyclonal
3.3471746 ABIN1874171 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3471746 ABIN3021659 IHC WB Rabbit IgG Log in to see Polyclonal
3.3471746 ABIN2476174 IF WB Mouse IgG2a AA 411-510 Log in to see 1D12
3.3471746 ABIN1175605 ICC IHC IP WB Rabbit IgG AA 59-239 Log in to see Polyclonal
3.3471746 ABIN3032330 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 470-499, Sumoylation Site Log in to see Polyclonal
3.3471746 ABIN562289 IF PLA ELISA WB Mouse IgG2a kappa AA 411-510, partial Log in to see 1D12
3.3471746 ABIN518988 IF ELISA WB Mouse IgG1 kappa AA 411-510, partial Log in to see 2B10
3.3471746 ABIN2404038 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3471746 ABIN927422 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PML antibody for Western Blotting

Similar anti-PML Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 44 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 10 Antibodies
In Vivo Imaging (IVI) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Western Blotting (WB) 119 Antibodies

General

Antigen Promyelocytic Leukemia (PML) Antibodies
Epitope N-Term
(18), (14), (14), (8), (7), (7), (6), (5), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(138), (44), (12), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(113), (41), (1)
Clonality
Polyclonal
Conjugate This PML antibody is un-conjugated
(7), (6), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(135), (59), (58), (46), (43), (38), (17), (16), (7), (6), (5), (4), (3), (3), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PML Antibody

Target Details PML Application Details Handling ProductDetails: References for anti-PML antibody (ABIN2780446) Images
Blocking Peptide Blocking peptide for this product available: ABIN981890
Sequence PSPSPSPTER APASEEEFQF LRCQQCQAEA KCPKLLPCLH TLCSGCLEAS
Specificity Isoform 1-14 specific
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against PML. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PML

Target Details PML

Product Details anti-PML Antibody Application Details Handling ProductDetails: References for anti-PML antibody (ABIN2780446) Images back to top
Antigen
Alternative Name PML (PML Antibody Abstract)
Background PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: MYL, PP8675, RNF71, TRIM19
Protein Interaction Partner: RPL11, RPL5, SUMO1, UBC, TP53, SUMO2, SUMO3, MDM2, RNF4, EGFR, NCOA2, SP100, DAXX, MAPK7, HDAC1, ATXN1, ATRX, ASF1A, EHMT2, HIRA, HTT, H3F3A, ATF7IP, PML, JAK1, EIF4E, GLIS2, UBE2I, smt3, HDAC11, HDAC6, LMNA, RNF111, CDK6, CDK2, CDK1, CASP8AP2, RBX1, SH3G
Protein Size: 435
Molecular Weight 48 kDa
Gene ID 5371
NCBI Accession NM_033247, NP_150250
UniProt P29590
Research Area Cancer
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus

Application Details

Product Details anti-PML Antibody Target Details PML Handling ProductDetails: References for anti-PML antibody (ABIN2780446) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 435 AA

Restrictions For Research Use only

Handling

Product Details anti-PML Antibody Target Details PML Application Details ProductDetails: References for anti-PML antibody (ABIN2780446) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PML antibody (ABIN2780446)

Product Details anti-PML Antibody Target Details PML Application Details Handling Images back to top
Background publications

Eun, Lee, Hong, Kim, Lee, Kim, Sun, Kim: "Hes6 controls cell proliferation via interaction with cAMP-response element-binding protein-binding protein in the promyelocytic leukemia nuclear body." in: The Journal of biological chemistry, Vol. 283, Issue 9, pp. 5939-49, 2008

Images

Product Details anti-PML Antibody Target Details PML Application Details Handling ProductDetails: References for anti-PML antibody (ABIN2780446) back to top
Supplier Images
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (N-Term) (ABIN2780446) WB Suggested Anti-PML Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (N-Term) (ABIN2780446) anti-Promyelocytic Leukemia (PML) (N-Term) antibody (Image 2)