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anti-Mouse (Murine) PML antibody for ELISA

Recommended PML Antibody (supplied by: Log in to see )

Antigen
Promyelocytic Leukemia (PML) Antibodies
  • 1200009E24Rik
  • AI661194
  • MYL
  • PML
  • PP8675
  • RGD1562602
  • RNF71
  • TRIM19
  • Trim19
Reactivity
Human, Mouse (Murine), Rat (Rattus)
110
25
7
1
Host
Rabbit
87
40
Clonality
Polyclonal
Conjugate
This PML antibody is un-conjugated
5
5
3
3
3
3
Application
ELISA, Western Blotting (WB)
105
45
39
38
24
20
13
7
6
3
3
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2457998
$ 433.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012121415 ABIN259796 IHC (p) IHC ELISA WB Mouse Log in to see Polyclonal
0.0012121415 ABIN347357 ELISA IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-PML Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 27 Antibodies 92 Antibodies 8 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Immunoprecipitation (IP) 8 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 40 Antibodies 9 Antibodies 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 1 Antibodies
Immunofluorescence (IF) 7 Antibodies 36 Antibodies 1 Antibodies

General

Antigen Promyelocytic Leukemia (PML) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(110), (25), (7), (1)
Host Rabbit
(87), (40)
Clonality
Polyclonal
Conjugate This PML antibody is un-conjugated
(5), (5), (3), (3), (3), (3)
Application ELISA, Western Blotting (WB)
(105), (45), (39), (38), (24), (20), (13), (7), (6), (3), (3), (3), (2), (1), (1), (1), (1)
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Product Details anti-PML Antibody

Target Details PML Application Details Handling Images
Purification Antibody is purified by peptide affinity chromatography method.
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PML.

Target Details PML

Product Details anti-PML Antibody Application Details Handling Images back to top
Antigen
Alternative Name PML (PML Antibody Abstract)
Background PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus.
Molecular Weight 59 kDa
Gene ID 5371
UniProt A4VCI9
Research Area Cancer
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway

Application Details

Product Details anti-PML Antibody Target Details PML Handling Images back to top
Application Notes PML antibody can be used for detection of PML by ELISA at 1:312500. PML antibody can be used for detection of PML by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only

Handling

Product Details anti-PML Antibody Target Details PML Application Details Images back to top
Format Lyophilized
Reconstitution Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store PML antibody at -20 °C.

Images

Product Details anti-PML Antibody Target Details PML Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (ABIN2457998) anti-Promyelocytic Leukemia (PML) antibody