|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||3 Antibodies||22 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||16 Antibodies||46 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Antigen||Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This PPP1R13B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-PPP1R13B AntibodyTarget Details PPP1R13B Application Details Handling Images
|Specificity||This antibody is directed against ASPP1.|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Characteristics||Concentration: 1.3 mg/mL (by UV absorbance at 280 nm)|
|Immunogen||Synthetic peptide corresponding to an internal sequence of human ASPP1|
Target Details PPP1R13BProduct Details anti-PPP1R13B Antibody Application Details Handling Images back to top
|Alternative Name||PPP1R13B (PPP1R13B Antibody Abstract)|
|Background||ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: ASPP1, Apoptosis-stimulating of p53 protein 1, KIAA0771, Protein phosphatase 1 regulatory subunit 13B|
Application DetailsProduct Details anti-PPP1R13B Antibody Target Details PPP1R13B Handling Images back to top
ELISA: 1/2,500 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PPP1R13B Antibody Target Details PPP1R13B Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|