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anti-Mouse (Murine) PPP1R13B antibody for Enzyme Immunoassay

Recommended PPP1R13B Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) Antibodies
  • AI449786
  • ASPP1
  • AW545810
  • p53BP2-like
  • p85
  • pp2ca1
  • ppp1r13b
  • si:ch211-253p18.1
  • Tp53bp2
  • Trp53bp2
  • wu:fd42g12
  • zgc:153820
Epitope
Internal Region
15
14
12
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
52
22
22
1
1
1
Host
Rabbit
51
1
Clonality
Polyclonal
Conjugate
This PPP1R13B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
39
22
11
4
3
2
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Catalog No. ABIN401394
$ 500.50
Plus shipping costs $45.00

Top referenced anti-PPP1R13B antibody for Enzyme Immunoassay

Similar anti-PPP1R13B Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 3 Antibodies 22 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 3 Antibodies
Western Blotting (WB) 12 Antibodies 42 Antibodies
Immunoprecipitation (IP) 6 Antibodies

General

Antigen Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) Antibodies
Epitope Internal Region
(15), (14), (12), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(52), (22), (22), (1), (1), (1)
Host Rabbit
(51), (1)
Clonality
Polyclonal
Conjugate This PPP1R13B antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(39), (22), (11), (4), (3), (2)
Pubmed 5 references available
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Product Details anti-PPP1R13B Antibody

Target Details PPP1R13B Application Details Handling References for anti-PPP1R13B Antibody (ABIN401394) Images
Specificity This antibody is directed against ASPP1.
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Affinity chromatography
Immunogen Synthetic peptide corresponding to an internal sequence of human ASPP1
Isotype IgG

Target Details PPP1R13B

Product Details anti-PPP1R13B Antibody Application Details Handling References for anti-PPP1R13B Antibody (ABIN401394) Images back to top
Antigen
Alternative Name PPP1R13B (PPP1R13B Antibody Abstract)
Background ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: ASPP1, Apoptosis-stimulating of p53 protein 1, KIAA0771, Protein phosphatase 1 regulatory subunit 13B
Gene ID 23368
NCBI Accession NP_056131
UniProt Q96KQ4
Pathways p53 Signaling

Application Details

Product Details anti-PPP1R13B Antibody Target Details PPP1R13B Handling References for anti-PPP1R13B Antibody (ABIN401394) Images back to top
Application Notes ELISA: 1/2,500 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PPP1R13B Antibody Target Details PPP1R13B Application Details References for anti-PPP1R13B Antibody (ABIN401394) Images back to top
Concentration 1.3 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-PPP1R13B Antibody (ABIN401394)

Product Details anti-PPP1R13B Antibody Target Details PPP1R13B Application Details Handling Images back to top
Background publications

Trigiante, Lu: "ASPP [corrected] and cancer." in: Nature reviews. Cancer, Vol. 6, Issue 3, pp. 217-26, 2006

Liu, Lu, Zhong: "ASPP--Apoptotic specific regulator of p53." in: Biochimica et biophysica acta, Vol. 1756, Issue 1, pp. 77-80, 2005

Liu, Lu, Zhang, Zhong, Gu, Zhang, Yang, Xin: "Downregulated mRNA expression of ASPP and the hypermethylation of the 5'-untranslated region in cancer cell lines retaining wild-type p53." in: FEBS letters, Vol. 579, Issue 7, pp. 1587-90, 2005

Slee, Lu: "The ASPP family: deciding between life and death after DNA damage." in: Toxicology letters, Vol. 139, Issue 2-3, pp. 81-7, 2003

Samuels-Lev, OConnor, Bergamaschi, Trigiante, Hsieh, Zhong, Campargue, Naumovski, Crook, Lu: "ASPP proteins specifically stimulate the apoptotic function of p53." in: Molecular cell, Vol. 8, Issue 4, pp. 781-94, 2001

Images

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Supplier Images
 image for anti-PPP1R13B antibody (Protein Phosphatase 1, Regulatory Subunit 13B) (Internal Region) (ABIN401394) anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (Internal Region) antibody