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|Antigen||Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies|
|Conjugate||This RRM2B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-RRM2B AntibodyTarget Details RRM2B Application Details Handling Images
|Specificity||This antibody is directed against RRM2B.|
Species reactivity (expected):Chimpanzee, Orangutan, Macaque, Porcine, Drosophila.
Species reactivity (tested):Human.
|Characteristics||Concentration: 0.82 mg/mL (by UV absorbance at 280nm)|
|Immunogen||Synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein|
Target Details RRM2BProduct Details anti-RRM2B Antibody Application Details Handling Images back to top
|Alternative Name||RRM2B / P53R2 (RRM2B Antibody Abstract)|
|Background||RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-ironcarboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein|
|Pathways||p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-RRM2B Antibody Target Details RRM2B Handling Images back to top
ELISA: 1/3,000. Western Blot: 1,0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RRM2B Antibody Target Details RRM2B Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|