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anti-Human RRM2B antibody for Enzyme Immunoassay

Recommended RRM2B Antibody (supplied by: Log in to see )

Antigen
Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
  • MTDPS8A
  • MTDPS8B
  • P53R2
  • p53r2
  • p53R2
  • RRM2B
Epitope
N-Term
17
16
15
12
11
3
3
2
2
2
2
1
1
1
Reactivity
Human
84
37
35
2
1
Host
Rabbit
79
5
Clonality
Polyclonal
Conjugate
This RRM2B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
65
40
16
11
11
7
4
1
1
Supplier
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Catalog No. ABIN401481
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008815228 ABIN500437 EIA IHC (p) WB Rabbit IgG AA 2-17, N-Term Log in to see Polyclonal 4

Top referenced anti-RRM2B antibody for Enzyme Immunoassay

Similar anti-RRM2B Antibodies

Application / Reactivity Human
ELISA 40 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 66 Antibodies

General

Antigen Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
Epitope N-Term
(17), (16), (15), (12), (11), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(84), (37), (35), (2), (1)
Host Rabbit
(79), (5)
Clonality
Polyclonal
Conjugate This RRM2B antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(65), (40), (16), (11), (11), (7), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-RRM2B Antibody

Target Details RRM2B Application Details Handling References for anti-RRM2B Antibody (ABIN401481) Images
Specificity This antibody is directed against RRM2B.
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee, Orangutan, Macaque, Porcine, Drosophila.
Species reactivity (tested):Human.
Purification Affinity Purified
Immunogen Synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein
Isotype IgG

Target Details RRM2B

Product Details anti-RRM2B Antibody Application Details Handling References for anti-RRM2B Antibody (ABIN401481) Images back to top
Antigen
Alternative Name RRM2B / P53R2 (RRM2B Antibody Abstract)
Background RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-ironcarboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein
Gene ID 50484
NCBI Accession NP_001165948
UniProt Q7LG56
Pathways p53 Signaling

Application Details

Product Details anti-RRM2B Antibody Target Details RRM2B Handling References for anti-RRM2B Antibody (ABIN401481) Images back to top
Application Notes ELISA: 1/3,000. Western Blot: 1,0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details References for anti-RRM2B Antibody (ABIN401481) Images back to top
Concentration 0.82 mg/mL (by UV absorbance at 280nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RRM2B Antibody (ABIN401481)

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling Images back to top
Background publications

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Images

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Supplier Images
 image for anti-RRM2B antibody (Ribonucleotide Reductase M2 B (TP53 Inducible)) (N-Term) (ABIN401481) anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (N-Term) antibody