anti-Human RRM2B antibody for ELISA

Recommended RRM2B Antibody (supplied by: Log in to see )

Antigen
Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
  • p53r2
  • RRM2B
  • MTDPS8A
  • MTDPS8B
  • P53R2
  • p53R2
  • ribonucleotide reductase M2 B (TP53 inducible)
  • rrm2b
  • RRM2B
  • Rrm2b
Epitope
AA 1-351
14
12
12
10
9
8
6
3
2
2
2
2
1
1
1
Reactivity
Human
88
44
41
4
3
3
2
2
2
2
1
1
1
1
1
1
Host
Mouse
83
5
Clonality
Monoclonal
Conjugate
This RRM2B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, ELISA (Detection), Western Blotting (WB)
67
38
17
14
13
8
4
2
2
1
1
Supplier
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Catalog No. ABIN1999939
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3186287 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4343019 ELISA ICC IF IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN565482 IF ELISA WB Mouse IgG2b kappa AA 3-84, partial Log in to see 6C1 2
1 ABIN964915 IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1104077 IF IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN964916 IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1031501 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1999943 IHC (p) ELISA WB Rabbit IgG AA 1-351 Log in to see Polyclonal
1 ABIN526296 ELISA WB Mouse IgG2a kappa AA 3-84, partial Log in to see 6C1
1 ABIN1820638 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1932173 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 128-157 Log in to see Polyclonal
1 ABIN1932176 ELISA WB FITC Rabbit IgG AA 128-157 Log in to see Polyclonal
1 ABIN1932177 ELISA WB PE Rabbit IgG AA 128-157 Log in to see Polyclonal
1 ABIN1932175 ELISA WB Biotin Rabbit IgG AA 128-157 Log in to see Polyclonal
1 ABIN1932174 ELISA WB APC Rabbit IgG AA 128-157 Log in to see Polyclonal
1 ABIN1932178 ELISA WB HRP Rabbit IgG AA 128-157 Log in to see Polyclonal
1 ABIN2579706 ELISA WB Rabbit IgG AA 127-157 Log in to see Polyclonal
1 ABIN2328809 ELISA WB Rabbit IgG AA 2-17, N-Term Log in to see Polyclonal
1 ABIN2354445 ELISA WB APC Rabbit IgG AA 127-157, Center, Internal Region Log in to see Polyclonal
1 ABIN2354453 ELISA WB Rabbit IgG AA 127-157, Center, Internal Region Log in to see Polyclonal

General

Antigen Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
Epitope AA 1-351
(14), (12), (12), (10), (9), (8), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(88), (44), (41), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(83), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RRM2B antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, ELISA (Detection), Western Blotting (WB)
(67), (38), (17), (14), (13), (8), (4), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-RRM2B Antibody

Target Details RRM2B Application Details Handling References for anti-RRM2B antibody (ABIN1999939) Images
Specificity Human RRM2B / P53R2
No cross-reactivity in ELISA with
E.coli cell lysate
No Cross-Reactivity Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RRM2B / P53R2 (rh RRM2B / P53R2, ABIN2005498 Q7LG56-1, Met 1-Phe 351,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human RRM2B / P53R2 protein (ABIN2005498)
Clone 04
Isotype IgG1

Target Details RRM2B

Product Details anti-RRM2B Antibody Application Details Handling References for anti-RRM2B antibody (ABIN1999939) Images back to top
Antigen
Alternative Name RRM2B (RRM2B Antibody Abstract)
Background Ribonucleoside reductase subunit M2B, also known as RRM2B or p53R2, is an enzyme belonging to the iron-dependent ribonucleotide reductase (RNR) enzyme family which is essential for DNA synthesis. Ribonucleotide reductase (RNR) is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides and plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. RRM2B is a phosphorylated protein. It is hypothesized that RRM2B activity can be regulated at the posttranslational level in response to DNA damage. RRM2B has previously been shown to be essential for the maintenance of mtDNA copy number and its candidacy for tumor suppression has been evaluated in several mutational analyses of different cancer types. However, the contribution of RRM2B to the DNA damage response has been questioned because its transcriptional induction upon DNA damage is not rapid enough for prompt DNA repair. Instead, ATM-mediated phosphorylation has been suggested to regulate the DNA repair activity of RRM2B posttranslationally. In addition, a defect in RRM2B can induce a mild muscle disease of adult onset through disturbance of mitochondrial homeostasis but that this defect does not appear to be oncogenic.,
UniProt Q7LG56-1
Pathways p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-RRM2B Antibody Target Details RRM2B Handling References for anti-RRM2B antibody (ABIN1999939) Images back to top
Application Notes WB: 20-30 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human RRM2B. The detection limit for Human RRM2B is approximately 0.039 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details References for anti-RRM2B antibody (ABIN1999939) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

References for anti-RRM2B antibody (ABIN1999939)

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling Images back to top
Background publications

Bourdon, Minai, Serre, Jais, Sarzi, Aubert, Chrétien, de Lonlay, Paquis-Flucklinger, Arakawa, Nakamura, Munnich, Rötig: "Mutation of RRM2B, encoding p53-controlled ribonucleotide reductase (p53R2), causes severe mitochondrial DNA depletion." in: Nature genetics, Vol. 39, Issue 6, pp. 776-80, 2007

Images

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling References for anti-RRM2B antibody (ABIN1999939) back to top
Supplier Images
Western Blotting (WB) image for anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 1-351) antibody (ABIN1999939) anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 1-351) antibody