Peptidylprolyl Isomerase B (Cyclophilin B) (PPIB) (AA 26-216) Peptide

Details for Product No. ABIN934947, Supplier: Log in to see
Protein Name
  • cypb
  • scylp
  • cyp-s1
  • PPIB
  • Ppib
  • ACYPI004891
  • AA408962
  • AA553318
  • AI844835
  • Cphn-2
  • Cphn2
  • CyP-20b
  • CypB
  • Scylp
  • CYP-S1
  • CYPB
  • OI9
  • SCYLP
  • cb87
  • sb:cb87
  • wu:fa97f08
  • zgc:73214
  • zgc:86796
  • peptidylprolyl isomerase B (cyclophilin B)
  • peptidylprolyl isomerase B
  • cyclophilin B
  • CYCLOPHILIN B protein
  • ppib
  • PPIB
  • Ppib
  • CC1G_03223
  • CYP-5
  • CYCLOPHILIN B
Protein Region
AA 26-216
Origin
Human
3
2
Source
Escherichia coli (E. coli)
Peptide Type
Recombinant
Biological Activity
Active
Application
SDS-PAGE (SDS)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Sequence MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
Characteristics Purified recombinant Human Cyclophilin B protein
Expression System: E.coli
Bioactivity: Specific activity is > 170 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.
Purity > 95 % pure
Endotoxin Level < 1.0 EU per µg of protein (determined by LAL method)
Background Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Cphn 2 protein, AA408962 protein, CYP S1 protein, Cyclophilin B protein, PPIB protein, MGC14109 protein, S cyclophilin., 26- 216aa protein, PPIase protein, Cphn2 protein, CyP 20b protein, SCYLP protein, AA553318 protein, AI844835 protein, Peptidyl prolyl cis trans isomerase B precursor protein, Peptidylprolyl isomerase B protein, Rotamase protein, CYPB protein, MGC2224 protein, peptidyl prolyl cis trans isomerase B protein, CYP-S1: Cyclophilin-B protein
Molecular Weight 21.2 kDa (192 AA)
Research Area Signaling, Cell Cycle, Apoptosis/Necrosis
Application Notes Cyclophilin B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Assay Procedure
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
    2. Add recombinant Cyclophilin B protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
    3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
    4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
    5. Record the increase in A410 nm for 20 minutes
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Supplied as a liquid in 20 mM Tris, pH 8.0, containing 20 mM NaCl, 0.0 mM DTT, and 10 % glycerol.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Supplier Images
SDS-PAGE (SDS) image for Peptidylprolyl Isomerase B (Cyclophilin B) (PPIB) (AA 26-216) peptide (ABIN934947) Figure annotation denotes ug of protein loaded and % gel used.