anti-Chimpanzee AKT antibody for Enzyme Immunoassay

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
Ser473
208
123
103
65
57
40
39
30
28
22
21
21
18
18
16
14
13
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chimpanzee, Human, Mouse (Murine), Rat (Rattus)
1221
646
502
63
58
56
46
35
33
30
18
8
8
6
5
5
3
3
2
2
1
1
1
1
1
Host
Mouse
884
380
12
4
2
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
54
41
39
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1040
552
455
252
183
174
119
82
67
32
26
15
13
11
11
4
3
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN5550837
$ 411.18
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Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
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Intracellular Staining (ICS) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope Ser473
(208), (123), (103), (65), (57), (40), (39), (30), (28), (22), (21), (21), (18), (18), (16), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chimpanzee, Human, Mouse (Murine), Rat (Rattus)
(1221), (646), (502), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(884), (380), (12), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(54), (41), (39), (38), (28), (25), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1040), (552), (455), (252), (183), (174), (119), (82), (67), (32), (26), (15), (13), (11), (11), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Specificity This phospho-specific monoclonal antibody reacts with Human and Mouse AKT pSer473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Cross-reactivity with AKT2 and AKT3 has not been determined.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Chimpanzee, Rat.
Species reactivity (tested):Human.
Characteristics Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase

Concentration: 1.02 mg/mL (by UV absorbance at 280 nm)
Purification Protein A Chromatography
Immunogen Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein
Clone 17F6-B11
Isotype IgG1

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
UniProt P31749
Research Area Kinases/Phosphatases, Protein Modifications, Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes ELISA: 1/20,000. Immunoprecipitation. Immunoflourescence: 1/500-1/3,000. Western blot: 1/500-1/3,000 (Expect a band approximately 56 kDa in size corresponding tophosphorylated AKT protein by in the appropriate cell lysate or extract). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 20 μg/mL (Nopre-treatment of sample is required).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Concentration 1.02 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Expiry Date 12 months