anti-Dog (Canine) AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
N-Term
225
131
115
65
57
51
39
37
36
32
22
22
18
16
13
12
12
12
11
11
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
1286
669
520
63
58
55
45
35
32
29
18
8
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
925
407
12
4
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
44
40
38
29
25
18
17
12
12
8
8
7
7
6
6
6
6
6
6
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
1080
562
484
259
209
175
129
87
67
33
26
15
13
11
11
7
4
4
3
3
3
1
1
1
1
1
1
1
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Catalog No. ABIN2792186
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2792187 WB Rabbit N-Term Log in to see Polyclonal 1
3.2182703 ABIN2705427 IHC WB Rabbit Center Log in to see Polyclonal
3.2182703 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
3.2182703 ABIN968206 IF IP IHC WB Mouse IgG1 AA 71-184 Log in to see 7-Akt-PKBalpha 5
3.2182703 ABIN968227 IF WB Mouse IgG2a AA 1-480 Log in to see 2-PKBa-Akt 2
3.2182703 ABIN968205 IF IP IHC WB Mouse IgG1 AA 71-184 Log in to see 7-Akt-PKBalpha 5
3.2182703 ABIN126858 EIA WB Mouse IgG1 AA 466-480 Log in to see 5C10
3.2182703 ABIN126853 EIA WB Mouse IgG1 pSer473 Log in to see 1-10E-007
3.2182703 ABIN968218 IF IP IHC WB Mouse IgG1, kappa Log in to see 55-PKBa-Akt 5
3.2182703 ABIN968217 IF IP IHC WB Mouse IgG1, kappa Log in to see 55-PKBa-Akt 2
3.2182703 ABIN126854 EIA WB Mouse IgG1 C-Term, Ser473 Log in to see 11A11
3.2182703 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5
3.2182703 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
3.2182703 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
3.2182703 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
3.2182703 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
3.2182703 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
3.2182703 ABIN2463692 ELISA WB Rabbit Log in to see Polyclonal
3.2182703 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 1
1 ABIN214085 IHC (p) WB Rabbit IgG AA 121-170 Log in to see Polyclonal

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope N-Term
(225), (131), (115), (65), (57), (51), (39), (37), (36), (32), (22), (22), (18), (16), (13), (12), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(1286), (669), (520), (63), (58), (55), (45), (35), (32), (29), (18), (8), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(925), (407), (12), (4), (2)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(54), (44), (40), (38), (29), (25), (18), (17), (12), (12), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(1080), (562), (484), (259), (209), (175), (129), (87), (67), (33), (26), (15), (13), (11), (11), (7), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN2792186) Images
Sequence RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK
Predicted Reactivity Cow: 86%, Dog: 100%, Goat: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rat: 100%, Sheep: 86%
Characteristics This is a rabbit polyclonal antibody against AKT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AKT1

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN2792186) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Alias Symbols: AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA
Protein Size: 480
Molecular Weight 56 kDa
Gene ID 207
NCBI Accession NM_005163, NP_005154
UniProt P31749
Research Area Cancer, Cell Cycle, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN2792186) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 480 AA

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN2792186) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AKT antibody (ABIN2792186)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Kim, Kim, Lee: "Expression of caveolin-1 is correlated with Akt-1 in colorectal cancer tissues." in: Experimental and molecular pathology, Vol. 80, Issue 2, pp. 165-70, 2006

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN2792186) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (N-Term) antibody (ABIN2792186) WB Suggested Anti-AKT1 Antibody
Positive Control: Lane 1:541 µg preterm baboon...