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anti-Human AKT antibody for Immunocytochemistry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
Center
186
136
90
88
54
39
38
38
26
25
21
20
18
16
16
15
15
14
13
13
11
10
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1138
529
396
43
41
34
33
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Rabbit
888
290
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
44
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
954
546
431
214
129
118
112
71
55
36
24
12
11
6
3
3
3
3
1
1
1
1
1
1
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Catalog No. ABIN2854922
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010120344 ABIN256750 ICC IF IHC (p) IHC WB Rabbit IgG Ser473 Log in to see EP2109Y
0.0010120344 ABIN213755 ICC IF IHC (p) ELISA WB Rabbit pSer473 Log in to see Polyclonal
0.0010120344 ABIN2964751 ICC WB Rabbit IgG pSer473 Log in to see 14-6
0.0010120344 ABIN2854921 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010120344 ABIN153477 ICC IF IHC (p) IHC ELISA WB Rabbit IgG AA 460-480 Log in to see Polyclonal
0.0010120344 ABIN1892231 ICC FACS IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0010120344 ABIN1892228 ICC FACS IHC ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal
0.0010120344 ABIN1892227 ICC FACS IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
0.0010120344 ABIN1892230 ICC FACS IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0010120344 ABIN1892229 ICC FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
0.0010120344 ABIN1821257 ICC WB Mouse IgG1 AA 5-30 Log in to see 1D4B
0.0010120344 ABIN604134 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010120344 ABIN1953099 DB ICC ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr308 Log in to see Polyclonal
0.0010120344 ABIN1953971 DB ICC ELISA APC Rabbit IgG Thr308 Log in to see Polyclonal
0.0010120344 ABIN1892225 ICC FACS IHC ELISA WB HRP Rabbit IgG C-Term Log in to see Polyclonal
0.0010120344 ABIN1956408 DB ICC ELISA FITC Rabbit IgG Thr308 Log in to see Polyclonal
0.0010120344 ABIN1955232 DB ICC ELISA Biotin Rabbit IgG Thr308 Log in to see Polyclonal
0.0010120344 ABIN1958744 DB ICC ELISA PE Rabbit IgG Thr308 Log in to see Polyclonal
0.0010120344 ABIN2886171 ICC Rabbit pThr308 Log in to see Polyclonal
0.0010120344 ABIN1860232 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 122-443 Log in to see Polyclonal

Similar anti-AKT Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 71 Antibodies
ELISA 512 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 36 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 124 Antibodies
Fluorescence Microscopy (FM) 3 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 59 Antibodies
Immunofluorescence (IF) 216 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 414 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope Center
(186), (136), (90), (88), (54), (39), (38), (38), (26), (25), (21), (20), (18), (16), (16), (15), (15), (14), (13), (13), (11), (10), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1138), (529), (396), (43), (41), (34), (33), (32), (20), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(888), (290), (14), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(44), (36), (35), (34), (27), (25), (10), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(954), (546), (431), (214), (129), (118), (112), (71), (55), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to AKT1 (v-akt murine thymoma viral oncogene homolog 1)
AKT1 antibody [N3C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human Akt1. The exact sequence is proprietary.
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Cellular Localization: Cytoplasm , Nucleus , Cell membrane
Molecular Weight 56 kDa
Gene ID 207
Research Area Cancer, Cell Cycle, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HA-AKT transfected HeLa cell , A431

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Concentration 0.17 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Center) (ABIN2854922) WB Image Sample (30 ug of whole cell lysate) A: A431 , 7.5% SDS PAGE antibody diluted...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Center) (ABIN2854922) WB Image Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfe...
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Center) (ABIN2854922) IHC-P Image Akt1 antibody detects Akt1 protein at nucleus on human ovarian carcinoma ...
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Center) (ABIN2854922) IHC-P Image Akt1 antibody detects Akt1 protein at nucleus on human breast cacinoma by...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Center) (ABIN2854922) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Akt1, ...