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anti-Human AKT antibody for Immunocytochemistry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
AA 2-480
188
136
90
87
54
38
38
37
26
25
21
21
20
19
16
15
15
15
14
13
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1162
542
410
44
41
37
34
32
20
18
11
6
5
4
2
2
2
1
1
Host
Mouse
894
311
14
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
45
39
35
34
26
25
10
10
5
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
975
554
446
223
142
120
117
71
62
36
25
11
11
5
3
3
3
3
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4899444
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010101626 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
0.0010101626 ABIN2854922 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0010101626 ABIN2854921 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010101626 ABIN2964751 ICC WB Rabbit IgG pSer473 Log in to see 14-6
0.0010101626 ABIN213755 ICC IF IHC (p) ELISA WB Rabbit pSer473 Log in to see Polyclonal
0.0010101626 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
0.0010101626 ABIN1892227 ICC FACS IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
0.0010101626 ABIN1892229 ICC FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
0.0010101626 ABIN1892228 ICC FACS IHC ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal
0.0010101626 ABIN1892230 ICC FACS IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0010101626 ABIN1892231 ICC FACS IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0010101626 ABIN1955232 DB ICC ELISA Biotin Rabbit IgG Thr308 Log in to see Polyclonal
0.0010101626 ABIN1956408 DB ICC ELISA FITC Rabbit IgG Thr308 Log in to see Polyclonal
0.0010101626 ABIN1953099 DB ICC ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr308 Log in to see Polyclonal
0.0010101626 ABIN1953971 DB ICC ELISA APC Rabbit IgG Thr308 Log in to see Polyclonal
0.0010101626 ABIN1892225 ICC FACS IHC ELISA WB HRP Rabbit IgG C-Term Log in to see Polyclonal
0.0010101626 ABIN1958744 DB ICC ELISA PE Rabbit IgG Thr308 Log in to see Polyclonal
0.0010101626 ABIN604134 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010101626 ABIN4278993 ICC IF IHC WB Rabbit AA 464-477 Log in to see Polyclonal 1
0.0010101626 ABIN1860232 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 122-443 Log in to see Polyclonal

Top referenced anti-AKT antibody for Immunocytochemistry

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
Dot Blot (DB) 1 Antibodies
ELISA 152 Antibodies
Enzyme Immunoassay (EIA) 21 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 31 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 96 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 25 Antibodies
Immunohistochemistry (IHC) 216 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 46 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Simple Western (SimWes) 2 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 2-480
(188), (136), (90), (87), (54), (38), (38), (37), (26), (25), (21), (21), (20), (19), (16), (15), (15), (15), (14), (13), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1162), (542), (410), (44), (41), (37), (34), (32), (20), (18), (11), (6), (5), (4), (2), (2), (2), (1), (1)
Host Mouse
(894), (311), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(45), (39), (35), (34), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
(975), (554), (446), (223), (142), (120), (117), (71), (62), (36), (25), (11), (11), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN4899444) Images
Specificity Detects human, mouse and rat Akt in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Akt1, Ser2-Ala480, Accession # P31749
Clone 281046
Isotype IgG2b

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN4899444) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN4899444) Images back to top
Application Notes Western Blot 0.2 μg/mL, Simple Western 2 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN4899444) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN4899444)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Tolvanen, Dash, Polianskyte-Prause, Dumont, Lehtonen: "Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes." in: Journal of cell science, Vol. 128, Issue 24, pp. 4588-600, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN4899444) back to top
Supplier Images
Simple Western (SimWes) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 hum...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human Akt Pan Specific by Western Blot. Western blot shows Recombinant H...
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Akt in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cance...
Immunocytochemistry (ICC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Akt in MDA-MB-231 Human Cell Line. Akt was detected in immersion fixed MDA-MB-231 hum...
Immunocytochemistry (ICC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Akt (pan) in 293T Human Cell Line. Akt pan specific (panels A and C) and Akt1 (E17K M...