anti-Human AKT antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 289-318
208
123
103
65
57
40
39
30
28
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
8
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1221
647
503
63
58
56
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
883
381
12
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
41
39
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1040
552
455
253
183
174
120
82
67
32
26
15
13
11
11
4
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN950327
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.368885 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
13.368885 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
13.368885 ABIN359291 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal
13.368885 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
13.368885 ABIN4279003 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
13.368885 ABIN498818 IHC (p) Rabbit pTyr326 Log in to see Polyclonal
13.368885 ABIN725180 IF (p) IHC (p) WB Mouse IgG AA 445-480 Log in to see Polyclonal
13.368885 ABIN214085 IHC (p) WB Rabbit IgG AA 121-170 Log in to see Polyclonal
13.368885 ABIN498224 IHC (p) Rabbit Log in to see Polyclonal
13.368885 ABIN615269 EIA IHC (p) WB Rabbit Log in to see Polyclonal
13.368885 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
13.368885 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
13.368885 ABIN213755 ICC IF IHC (p) ELISA WB Rabbit pSer473 Log in to see Polyclonal
13.368885 ABIN213898 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
13.368885 ABIN213759 IHC (p) ELISA WB Rabbit pThr308 Log in to see Polyclonal
13.368885 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 1
13.368885 ABIN4278998 ELISA IHC IHC (p) IP WB Mouse IgG1 Log in to see 3A3
13.368885 ABIN443649 IHC (p) IHC Rabbit C-Term, pSer473 Log in to see Polyclonal
13.368885 ABIN4956766 ELISA IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal
13.368885 ABIN5557411 IF (cc) IHC (p) WB Rabbit IgG pSer473 Log in to see 12A1

Top referenced anti-AKT antibody for Immunohistochemistry (Paraffin-embedded Sections)

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General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 289-318
(208), (123), (103), (65), (57), (40), (39), (30), (28), (22), (21), (21), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (8), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1221), (647), (503), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(883), (381), (12), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(54), (41), (39), (38), (28), (25), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1040), (552), (455), (253), (183), (174), (120), (82), (67), (32), (26), (15), (13), (11), (11), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN950327) Images
Specificity This antibody reacts to AKT1.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 289-318 amino acids from human AKT1
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN950327) Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN950327) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN950327) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-AKT antibody (ABIN950327)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Zhao, Liu, Fang, Kim, Chen: "Vascular endothelial growth factor receptor 1 contributes to Escherichia coli K1 invasion of human brain microvascular endothelial cells through the phosphatidylinositol 3-kinase/Akt signaling pathway." in: Infection and immunity, Vol. 78, Issue 11, pp. 4809-16, 2010

Sanematsu, Hirashima, Laurin, Takii, Nishikimi, Kitajima, Ding, Noda, Murata, Tanaka, Masuko, Suda, Meno, C��té, Nagasawa, Fukui: "DOCK180 is a Rac activator that regulates cardiovascular development by acting downstream of CXCR4." in: Circulation research, Vol. 107, Issue 9, pp. 1102-5, 2010

Nicodemus, Law, Radulescu, Luna, Kolachana, Vakkalanka, Rujescu, Giegling, Straub, McGee, Gold, Dean, Muglia, Callicott, Tan, Weinberger: "Biological validation of increased schizophrenia risk with NRG1, ERBB4, and AKT1 epistasis via functional neuroimaging in healthy controls." in: Archives of general psychiatry, Vol. 67, Issue 10, pp. 991-1001, 2010

Treekitkarnmongkol, Suthiphongchai: "High expression of ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K." in: World journal of gastroenterology : WJG, Vol. 16, Issue 32, pp. 4047-54, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN950327) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 289-318) (ABIN950327) AKT1 Antibody (Thr308) flow cytometric analysis of MDA-MB435 cells (right histogram) ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 289-318) (ABIN950327) AKT1 antibody (Thr308) immunohistochemistry analysis in formalin fixed and paraffin e...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 289-318) (ABIN950327) AKT1 (Thr308) Antibody western blot analysis in MDA-MB435 cell line lysates (35µg/lan...