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anti-Monkey AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
pThr308, Internal Region
186
136
89
88
54
39
38
37
26
25
21
20
18
16
16
15
15
14
13
13
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Monkey
1138
528
395
43
41
34
32
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Mouse
889
289
14
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is conjugated to Biotin
43
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
954
545
430
215
129
119
111
71
56
36
24
12
11
6
3
3
3
3
1
1
1
1
1
1
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Catalog No. ABIN1043753
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
0.13398378 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
0.13398378 ABIN968951 ELISA WB Mouse IgG1 Log in to see 3A3 3
0.13398378 ABIN964560 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
0.13398378 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
0.13398378 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
0.13398378 ABIN964562 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
0.13398378 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
0.13398378 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
0.13398378 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
0.13398378 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
0.13398378 ABIN2704460 IHC WB Rabbit C-Term, pTyr474 Log in to see Polyclonal
0.13398378 ABIN2707319 IHC WB Rabbit N-Term Log in to see Polyclonal
0.13398378 ABIN2167707 ELISA WB Mouse IgG1 Log in to see 3A3
0.13398378 ABIN1583853 WB Mouse IgG1 Log in to see 3A3
0.13398378 ABIN1105271 EIA WB Mouse IgG1 Log in to see 3A3
0.13398378 ABIN3183233 ELISA IF IHC WB Rabbit IgG Ser13 Log in to see Polyclonal
0.13398378 ABIN3182568 ELISA IHC WB Rabbit IgG pTyr474 Log in to see Polyclonal
0.13398378 ABIN3181908 ELISA IHC WB Rabbit IgG pThr308 Log in to see Polyclonal
0.13398378 ABIN1845827 ELISA WB Mouse IgG1 Log in to see

Top referenced anti-AKT antibody for Western Blotting

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
Dot Blot (DB) 1 Antibodies
ELISA 149 Antibodies
Enzyme Immunoassay (EIA) 21 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 210 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 41 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies
Western Blotting (WB) 337 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pThr308, Internal Region
(186), (136), (89), (88), (54), (39), (38), (37), (26), (25), (21), (20), (18), (16), (16), (15), (15), (14), (13), (13), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Monkey
(1138), (528), (395), (43), (41), (34), (32), (32), (20), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Host Mouse
(889), (289), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is conjugated to Biotin
(43), (36), (35), (34), (27), (25), (10), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(954), (545), (430), (215), (129), (119), (111), (71), (56), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1043753) Images
Specificity Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Characteristics Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Immunogen Type: Peptide
Clone 18F3-H11
Isotype IgG1 kappa

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN1043753) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN1043753) Images back to top
Application Notes Biotin Conjugated Anti-AKT pT308 is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
Comment

Gene Name: AKT1

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN1043753) Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN1043753)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1043753) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308) (Biotin) (ABIN1043753) Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST t...
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308) (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 2)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308) (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 3)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308) (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 4)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308) (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 5)