anti-Mouse (Murine) AKT antibody for Immunocytochemistry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 2-480, pan
188
123
85
65
56
39
32
30
28
21
21
20
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1136
636
493
63
58
56
46
35
33
30
18
8
8
5
5
5
3
3
3
2
1
1
1
1
Host
Mouse
856
322
11
4
2
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
48
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
964
527
435
220
158
149
116
67
55
33
26
15
11
11
4
3
3
3
3
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN4899444
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.67279 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
1 ABIN2854921 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2964751 ICC WB Rabbit IgG pSer473 Log in to see 14-6
1 ABIN4279002 ELISA ICC IF DyLight 488 Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4279006 ELISA FACS ICC IF IHC IHC (p) WB Biotin Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN4279001 ELISA FLISA ICC IF DyLight 488 Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
1 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN213755 ICC IF IHC (p) ELISA WB Rabbit pSer473 Log in to see Polyclonal
1 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6
1 ABIN4279049 ELISA ICC IF IP WB Rabbit IgG C-Term Log in to see C20-A
1 ABIN4278991 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4902874 FACS ICC IF IHC IP WB Rabbit IgG Log in to see
1 ABIN2738220 ICC IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2886171 ICC IHC IP WB Rabbit pThr308 Log in to see Polyclonal
1 ABIN4902869 FACS ICC IF IHC IP WB Rabbit IgG pan Log in to see
1 ABIN5550451 ELISA FACS ICC IF WB Mouse IgG2a kappa AA 1-480 Log in to see 6D5
1 ABIN5550450 ELISA FACS ICC IF WB Mouse IgG2a kappa AA 1-480 Log in to see 6D5

Top referenced anti-AKT antibody for Immunocytochemistry

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Application / Reactivity Rat (Rattus)
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ELISA 171 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 219 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 91 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 2-480, pan
(188), (123), (85), (65), (56), (39), (32), (30), (28), (21), (21), (20), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1136), (636), (493), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(856), (322), (11), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(48), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
(964), (527), (435), (220), (158), (149), (116), (67), (55), (33), (26), (15), (11), (11), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN4899444) Images
Specificity Detects human, mouse and rat Akt in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Akt1, Ser2-Ala480, Accession # P31749
Clone 281046
Isotype IgG2b

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN4899444) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Gene ID 207
UniProt P31749
Research Area Kinases/Phosphatases, Cell Cycle, Apoptosis/Necrosis, Cancer
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN4899444) Images back to top
Application Notes Western Blot 0.2 μg/mL
Simple Western 2 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN4899444) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-AKT antibody (ABIN4899444)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Tolvanen, Dash, Polianskyte-Prause, Dumont, Lehtonen: "Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes." in: Journal of cell science, Vol. 128, Issue 24, pp. 4588-600, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN4899444) back to top
Supplier Images
Simple Western (SimWes) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 hum...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human Akt Pan Specific by Western Blot. Western blot shows Recombinant H...
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Akt in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cance...
Immunocytochemistry (ICC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Akt in MDA-MB-231 Human Cell Line. Akt was detected in immersion fixed MDA-MB-231 hum...
Immunocytochemistry (ICC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Akt (pan) in 293T Human Cell Line. Akt pan specific (panels A and C) and Akt1 (E17K M...