anti-Rat (Rattus) BIM antibody for Immunohistochemistry

Recommended BIM Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • LOC100345483
  • BCL2L11
  • Bcl2l11
Reactivity
Human, Mouse (Murine), Rat (Rattus)
329
235
207
9
6
6
4
3
3
1
1
Host
Rabbit
280
29
16
9
7
Clonality
Polyclonal
Conjugate
This BIM antibody is conjugated to Atto 390
16
14
11
9
9
9
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
276
138
113
79
70
44
33
28
17
7
5
4
3
2
2
2
2
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Catalog No. ABIN2485927
$ 397.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.921165 ABIN2485928 IHC WB Atto 488 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485929 IHC WB Atto 565 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485930 IHC WB Atto 594 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485931 IHC WB Atto 633 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485932 IHC WB Atto 655 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485933 IHC WB Atto 680 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485934 IHC WB Atto 700 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485935 IHC WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485936 IHC WB APC Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485937 IHC WB Biotin Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485938 IHC WB FITC Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485939 IHC WB HRP Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485940 IHC WB PE-Atto 594 Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485941 IHC WB PerCP Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485942 IHC WB PE Rabbit Log in to see Polyclonal 2
8.921165 ABIN2485943 IHC WB Streptavidin Rabbit Log in to see Polyclonal 2
8.921165 ABIN4284636 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 1
8.921165 ABIN2707367 IHC WB Rabbit N-Term Log in to see Polyclonal
8.921165 ABIN2705596 IC IF IHC WB Rabbit pSer69 Log in to see Polyclonal
8.921165 ABIN1870017 IF IHC Rabbit IgG pSer69 Log in to see Polyclonal

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(329), (235), (207), (9), (6), (6), (4), (3), (3), (1), (1)
Host Rabbit
(280), (29), (16), (9), (7)
Clonality
Polyclonal
Conjugate This BIM antibody is conjugated to Atto 390
(16), (14), (11), (9), (9), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(276), (138), (113), (79), (70), (44), (33), (28), (17), (7), (5), (4), (3), (2), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-BIM Antibody

Target Details BIM Application Details Handling ProductDetails: References for anti-BIM antibody (ABIN2485927) Images
Specificity Detects ~23 kDa.
Purification Protein A Purified
Immunogen Internal central amino acids of human Bim

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling ProductDetails: References for anti-BIM antibody (ABIN2485927) Images back to top
Antigen
Alternative Name BIM/BOD (BCL2L11 Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1 ,2).
Gene ID 10018
NCBI Accession NP_619527
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling ProductDetails: References for anti-BIM antibody (ABIN2485927) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SPC-113 was sufficient for detection of Bim in 20 μg of K562 and A549 cell lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details ProductDetails: References for anti-BIM antibody (ABIN2485927) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

ProductDetails: References for anti-BIM antibody (ABIN2485927)

Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
Background publications

OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling ProductDetails: References for anti-BIM antibody (ABIN2485927) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (Atto 390) (ABIN2485927) Immunohistochemistry analysis using Rabbit Anti-BIM Polyclonal Antibody . Tissue: ski...
Western Blotting (WB) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (Atto 390) (ABIN2485927) Western blot analysis of Human K562 (Left) and A549 (Right) whole cell lysates showin...