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anti-Mouse (Murine) Cyclin A antibody for Western Blotting

Recommended Cyclin A Antibody (supplied by: Log in to see )

Antigen
Cyclin A2 (CCNA2) Antibodies
  • AA408589
  • cb671
  • Ccn-1
  • ccn1
  • Ccn1
  • CCN1
  • ccna
  • Ccna
  • CCNA
  • ccna1
  • ccna2
  • chunp6924
  • Cyca
  • CYCA
  • CycA2
  • Cyclin-A
  • Cyclin-A2
  • cyclinA
  • wu:fi36f03
Reactivity
Human, Mouse (Murine), Rat (Rattus)
253
92
71
36
22
22
18
5
3
3
2
2
2
2
2
1
Host
Mouse
190
105
1
Clonality
Monoclonal
Conjugate
This Cyclin A antibody is conjugated to Biotin
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
229
76
67
60
54
49
46
38
26
5
4
3
2
2
1
1
Supplier
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Catalog No. ABIN2853583
$ 392.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2792174 WB Rabbit C-Term Log in to see Polyclonal 1
0.13341247 ABIN567778 WB Mouse IgG2a Log in to see E23-1 1
0.13341247 ABIN567779 IP WB Mouse IgG2a Log in to see E67-1 2
0.13341247 ABIN1533247 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2855544 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.13341247 ABIN3043070 ICC WB Mouse IgG2a Log in to see CY-A1
0.13341247 ABIN2664910 WB Mouse IgG2a Log in to see E23-1 3
0.13341247 ABIN1871530 IF WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2855543 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.13341247 ABIN3184138 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13341247 ABIN926002 WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN1848492 ICC IF IHC ELISA WB Rabbit Internal Region Log in to see Polyclonal
0.0011728023 ABIN181287 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 2
0.0011728023 ABIN1106864 IF WB Mouse IgG2a Log in to see CY-28 1
0.0011728023 ABIN670281 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.0011728023 ABIN265377 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN1742579 IP WB Mouse IgG2a Log in to see 3
0.0011728023 ABIN121516 EIA IHC (p) WB Rabbit Log in to see Polyclonal 3
0.0011728023 ABIN269752 IHC (p) IP IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1583849 ICC ELISA WB Mouse IgG2a Log in to see CY-28

Top referenced anti-Cyclin A antibody for Western Blotting

Similar anti-Cyclin A Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 197 Antibodies 71 Antibodies 36 Antibodies
Immunoprecipitation (IP) 52 Antibodies 24 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 77 Antibodies 17 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 48 Antibodies 17 Antibodies 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 46 Antibodies 26 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies

General

Antigen Cyclin A2 (CCNA2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(253), (92), (71), (36), (22), (22), (18), (5), (3), (3), (2), (2), (2), (2), (2), (1)
Host Mouse
(190), (105), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin A antibody is conjugated to Biotin
(8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(229), (76), (67), (60), (54), (49), (46), (38), (26), (5), (4), (3), (2), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-Cyclin A Antibody

Target Details Cyclin A Application Details Handling References for anti-Cyclin A Antibody (ABIN2853583) Images
Specificity This antibody reacts with Cyclin A (55 kDa).
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Immunogen Bovine recombinant Cyclin A.
Clone E67-1
Isotype IgG2a

Target Details Cyclin A

Product Details anti-Cyclin A Antibody Application Details Handling References for anti-Cyclin A Antibody (ABIN2853583) Images back to top
Antigen
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background Cyclin A is a 60 kDa cyclin that binds both cdk2 and p34cdc2 (cdk1), giving two distinct cyclin A kinase activities, one appearing in G1/S phase, the other in G2/M. The level of cyclin A protein peaks in S-phase, and the activity of the cdk2-cyclin A complexes is essential for DNA replication and progression through S phase. Cyclin A is thus a marker for actively proliferating cells and for cells in S-phase. In normal cells, cdk2-cyclin A complexes associate with PCNA and p21CIPP1/WAF1. In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcrIPPtion factors DRT F1 and E2F, and retinoblastoma protein, p110.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Gene ID 890
NCBI Accession NP_001228
UniProt P20248
Research Area Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling

Application Details

Product Details anti-Cyclin A Antibody Target Details Cyclin A Handling References for anti-Cyclin A Antibody (ABIN2853583) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details References for anti-Cyclin A Antibody (ABIN2853583) Images back to top
Concentration 20 µg/mL
Buffer PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-Cyclin A Antibody (ABIN2853583)

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling Images back to top
Background publications

Di Bartolo, Cannon, Liu, Renne, Chadburn, Boshoff, Cesarman: "KSHV LANA inhibits TGF-beta signaling through epigenetic silencing of the TGF-beta type II receptor." in: Blood, Vol. 111, Issue 9, pp. 4731-40, 2008

Ciarallo, Subramaniam, Hung, Lee, Kotchetkov, Sandhu, Milic, Slingerland: "Altered p27(Kip1) phosphorylation, localization, and function in human epithelial cells resistant to transforming growth factor beta-mediated G(1) arrest." in: Molecular and cellular biology, Vol. 22, Issue 9, pp. 2993-3002, 2002

Sandhu, Donovan, Bhattacharya, Stampfer, Worland, Slingerland: "Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells." in: Oncogene, Vol. 19, Issue 47, pp. 5314-23, 2000

Sandhu, Peehl, Slingerland: "p16INK4A mediates cyclin dependent kinase 4 and 6 inhibition in senescent prostatic epithelial cells." in: Cancer research, Vol. 60, Issue 10, pp. 2616-22, 2000

Slingerland, Hengst, Pan, Alexander, Stampfer, Reed: "A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells." in: Molecular and cellular biology, Vol. 14, Issue 6, pp. 3683-94, 1994

Dveksler, Dieffenbach, Cardellichio, McCuaig, Pensiero, Jiang, Beauchemin, Holmes: "Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59." in: Journal of virology, Vol. 67, Issue 1, pp. 1-8, 1993

Images

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling References for anti-Cyclin A Antibody (ABIN2853583) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin A antibody (Cyclin A2)  (Biotin) (ABIN2853583) anti-Cyclin A2 (CCNA2) antibody (Biotin)