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anti-Human Cyclin D1 antibody for Immunocytochemistry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Epitope
C-Term, AA 250-295
79
25
19
14
13
12
10
10
9
9
8
6
6
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
448
182
173
46
16
13
13
11
9
8
4
3
2
2
2
1
1
1
1
Host
Rabbit
311
188
1
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
22
19
9
9
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
401
191
140
108
107
104
103
73
71
12
8
7
6
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN153471
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010193537 ABIN302101 ICC FACS IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1 2
0.0010193537 ABIN574432 ICC IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1
0.0010193537 ABIN3043639 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see DCS-6
0.0010193537 ABIN1501952 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see CYD1
0.0010193537 ABIN256781 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term, AA 245-295 Log in to see EPR2241
0.0010193537 ABIN189278 ICC IF IHC (p) IP IHC WB Rabbit IgG N-Term, AA 1-50 Log in to see EP272Y
0.0010193537 ABIN2471067 ICC FACS IP IHC ELISA WB Mouse IgG1 Log in to see CD1-1
0.0010193537 ABIN609471 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see
0.0010193537 ABIN211137 Func ICC IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010193537 ABIN1858284 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-295 Log in to see Polyclonal
0.0010193537 ABIN1723570 ICC IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1
0.0010193537 ABIN4301539 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301540 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301563 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301564 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301565 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301566 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301567 FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2a kappa Log in to see SPM587
0.0010193537 ABIN4301568 FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a kappa Log in to see SPM587

Top referenced anti-Cyclin D1 antibody for Immunocytochemistry

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 64 Antibodies 166 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 7 Antibodies
Flow Cytometry (FACS) 52 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 58 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 66 Antibodies
Immunoprecipitation (IP) 66 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 156 Antibodies
Blocking Reagent (BR) 2 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope C-Term, AA 250-295
(79), (25), (19), (14), (13), (12), (10), (10), (9), (9), (8), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(448), (182), (173), (46), (16), (13), (13), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(311), (188), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(22), (19), (9), (9), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(401), (191), (140), (108), (107), (104), (103), (73), (71), (12), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN153471) Images
Specificity Cyclin D1 (SP4)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Tissue culture supernatant
Immunogen Synthetic peptide from C-terminus of human cyclin D1 (within amino acids 250-295 of P24385 - UniProt).
Clone SP4
Isotype IgG

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN153471) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions inassociation with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putativeproto-oncogene overexpressed in a wide variety of human neoplasmsincluding mantle cell lymphomas (MCL). Alternate Names: Anti-B cell ccl/lymphoma 1 antibody, Anti-B cell leukemia 1 antibody, Anti-BCL1antibody, Anti-BCL1 oncogene antibody, Anti-CCND 1 antibody, Anti-CCND1 antibody,Anti-CCND1 protein antibody, Anti-CCND1/IGHG1 fusion gene antibody,Anti-D11S287E antibody, Anti-G1/S specific cyclin D1 antibody, Anti-Parathyroidadenomatosis 1 antibody, Anti-PRAD1 antibody, Anti-PRAD1 oncogene antibody,Anti-U21B31 antibody.
Gene Symbol: CCND1
Gene ID 595, 12443, 58919
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN153471) Images back to top
Application Notes This antibody can be used in Western Blot and Immunohistochemistry-Paraffin. For IHC, staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. In Western Blot, a 33kDa band is seen. *Optimal dilutions should be determined by the end user.Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24599075)
Recommended dilutions: Immunocytochemistry/Immunofluorescence, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Western Blot 1:100-1:2000
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN153471) Images back to top
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

References for anti-Cyclin D1 Antibody (ABIN153471)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Product cited in:

Cheuk, Wong, Wong, Chan: "Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody." in: The American journal of surgical pathology, Vol. 28, Issue 6, pp. 801-7, 2004

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN153471) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Cyclin D1 antibody (CCND1) (C-Term) (ABIN153471) anti-Cyclin D1 (CCND1) (AA 250-295), (C-Term) antibody