You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) Cyclin D1 antibody for Flow Cytometry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Reactivity
Human, Rat (Rattus)
363
131
120
16
13
13
11
9
8
4
3
2
2
2
1
1
1
Host
Mouse
300
113
1
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
21
16
6
6
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
322
162
98
65
64
55
40
29
29
12
8
7
6
3
3
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN302101
$ 288.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010522496 ABIN2471067 ICC FACS IP IHC ELISA WB Mouse IgG1 Log in to see CD1-1
0.0010522496 ABIN284021 FACS IF IP IHC WB Mouse IgG1 kappa Log in to see DCS-6
0.0010522496 ABIN1723570 ICC IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1
0.0010522496 ABIN3026634 FACS IF IHC WB Mouse IgG2a, kappa Log in to see DCS-6
0.0010522496 ABIN3200126 ELISA FACS ICC IHC IP WB Mouse IgG1 Log in to see CD1-1
0.0010522496 ABIN3026631 FACS IF PE Mouse IgG2a, kappa Log in to see DCS-6
0.0010522496 ABIN3026632 FACS IF PE Mouse IgG2a, kappa Log in to see CCND1-809
0.0010522496 ABIN2473139 IHC (fro) FACS IP IHC (p) ELISA WB Mouse IgG1 Log in to see CD1-1 2
0.0010522496 ABIN3026636 FACS IF IHC WB Mouse IgG2a, kappa Log in to see DCS-6
0.0010522496 ABIN3024574 FACS IF IHC WB Mouse IgG2a, kappa Log in to see SPM587
0.0010522496 ABIN3037874 ICC FACS IF IP IHC ELISA WB Mouse IgG1 Log in to see
0.0010522496 ABIN3024575 FACS IF IHC WB Mouse IgG2a, kappa Log in to see SPM587

Top referenced anti-Cyclin D1 antibody for Flow Cytometry

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 37 Antibodies 138 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 100 Antibodies
Blocking Reagent (BR) 2 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Rat (Rattus)
(363), (131), (120), (16), (13), (13), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(300), (113), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(21), (16), (6), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(322), (162), (98), (65), (64), (55), (40), (29), (29), (12), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN302101) Images
Specificity The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Purified cyclin D1 protein
Clone CD1-1
Isotype IgG1

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN302101) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN302101) Images back to top
Application Notes Flow Cytometry:
Application note: Membrane permeabilization is required.
Immunoprecipitation: Recommended dilution: 1 µg/mL
Western Blotting: Recommended dilution: 1 µg/mL
Immunohistochemistry (paraffin sections)
Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
Immunohistochemistry (frozen sections) Recommended dilution: 2 µg/mL
Positive tissue: colon.
Immunocytochemistry: Recommended dilution: 1 µg/mL

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN302101) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-Cyclin D1 Antibody (ABIN302101)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Background publications

Diehl, Cheng, Roussel, Sherr: "Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization." in: Genes & development, Vol. 12, Issue 22, pp. 3499-511, 1999

Diehl, Zindy, Sherr: "Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway." in: Genes & development, Vol. 11, Issue 8, pp. 957-72, 1997