|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||37 Antibodies||37 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||13 Antibodies||10 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||19 Antibodies|
|Immunofluorescence (IF)||21 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||44 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies|
|Immunoprecipitation (IP)||24 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Western Blotting (WB)||100 Antibodies|
|Antigen||Cyclin D1 (CCND1) Antibodies|
|Epitope||full length Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cyclin D1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-Cyclin D1 AntibodyTarget Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN967539) Images
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human Full-length Cyclin D1 Recombinant Protein|
Target Details Cyclin D1Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN967539) Images back to top
|Alternative Name||Cyclin D1 (CCND1 Antibody Abstract)|
|Background||Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.|
|Molecular Weight||36 kDa|
|Research Area||Cancer, Cell Cycle|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN967539) Images back to top
Related Products: ABIN968585, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN967539) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-Cyclin D1 Antibody (ABIN967539)Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
|Product cited in:||
Darzynkiewicz, Gong, Juan et al.: "Cytometry of cyclin proteins." in: Cytometry, Vol. 25, Issue 1, pp. 1-13, 1997 (PubMed).
Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).
Lukas, Pagano, Staskova et al.: "Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines." in: Oncogene, Vol. 9, Issue 3, pp. 707-18, 1994 (PubMed).
de Boer, Schuuring, Dreef et al.: "Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma." in: Blood, Vol. 86, Issue 7, pp. 2715-23, 1995 (PubMed).
ImagesProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN967539) back to top