You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Huntingtin antibody for ELISA

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • AI256365
  • C430023I11Rik
  • CG9995
  • dHtt
  • dhtt
  • Dmel\\CG9995
  • HD
  • hd
  • Hd
  • Hdh
  • Hsap\\HD
  • htt
  • HTT
  • Htt
  • huntington
  • it15
  • IT15
  • SLC6A4
  • ZHD
Epitope
AA 81-191
13
13
12
7
6
6
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
124
47
35
7
Host
Mouse
67
60
2
1
Clonality
Monoclonal
Conjugate
This Huntingtin antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
96
65
42
21
20
18
11
11
11
8
3
3
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN394366
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30628014 ABIN516379 ELISA WB Mouse IgG2a kappa AA 81-190, partial Log in to see 1H6 1
0.30628014 ABIN516380 ELISA WB Mouse IgG2b kappa AA 81-190, partial Log in to see 3F9
0.30628014 ABIN516378 ELISA WB Mouse IgG2a kappa AA 81-190, partial Log in to see 4G6
0.30628014 ABIN516381 ELISA WB Mouse IgG2a kappa AA 81-190, partial Log in to see 3D6
0.30628014 ABIN516382 IHC (p) ELISA WB Mouse IgG2a kappa AA 81-190, partial Log in to see 3F1
0.30628014 ABIN561241 IF ELISA WB Mouse IgG1 kappa AA 81-190, partial Log in to see 2E10
0.0012155735 ABIN1450584 IHC ELISA Rabbit IgG N-Term Log in to see Polyclonal 3
0.0012155735 ABIN100604 IHC ELISA WB Rabbit IgG AA 416-424, pSer421 Log in to see Polyclonal
0.0012155735 ABIN2470221 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012155735 ABIN214338 IHC (p) ELISA WB Rabbit pSer421 Log in to see Polyclonal
0.0012155735 ABIN626061 IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see 3F1
0.0012155735 ABIN1735629 IHC (p) ELISA WB Rabbit IgG pSer421 Log in to see Polyclonal
0.0012155735 ABIN228545 ICC IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 AA 181-810 Log in to see 2Q75
0.0012155735 ABIN201065 ELISA Rabbit pSer421 Log in to see Polyclonal
0.0012155735 ABIN2471198 IHC ELISA WB Mouse IgG2a kappa Log in to see 3F1
0.0012155735 ABIN1450323 IHC ELISA Rabbit IgG pSer421, N-Term Log in to see Polyclonal
0.0012155735 ABIN3185117 ELISA IHC Rabbit IgG Ser47 Log in to see Polyclonal
0.0012155735 ABIN3182875 ELISA IHC Rabbit IgG pSer421 Log in to see Polyclonal
0.0012155735 ABIN1723674 IHC (p) ELISA WB Mouse IgG2a kappa AA 81-191 Log in to see 3F1
0.0012155735 ABIN516383 ELISA WB Mouse IgG2b kappa AA 1524-1627, partial Log in to see 2A7

Top referenced anti-Huntingtin antibody for ELISA

Similar anti-Huntingtin Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 64 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 96 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 81-191
(13), (13), (12), (7), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(124), (47), (35), (7)
Host Mouse
(67), (60), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Huntingtin antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(96), (65), (42), (21), (20), (18), (11), (11), (11), (8), (3), (3), (2)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN394366) Images
Specificity HD (NP_002102, 81 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen HD (NP_002102, 81 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4G6
Isotype IgG2a kappa

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin Antibody (ABIN394366) Images back to top
Antigen
Alternative Name HD (HTT Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 347860 DA
Gene ID 12406
NCBI Accession NP_002102
Research Area Translation Factors, Transcription Factors, Signaling, Cell Structure
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin Antibody (ABIN394366) Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.

Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin Antibody (ABIN394366) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-Huntingtin Antibody (ABIN394366)

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Background publications

Aziz, Pijl, Frölich, Snel, Streefland, Roelfsema, Roos: "Systemic energy homeostasis in Huntington's disease patients." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 81, Issue 11, pp. 1233-7, 2010

Godin, Poizat, Hickey, Maschat, Humbert: "Mutant huntingtin-impaired degradation of beta-catenin causes neurotoxicity in Huntington's disease." in: The EMBO journal, Vol. 29, Issue 14, pp. 2433-45, 2010

Olshina, Angley, Ramdzan, Tang, Bailey, Hill, Hatters: "Tracking mutant huntingtin aggregation kinetics in cells reveals three major populations that include an invariant oligomer pool." in: The Journal of biological chemistry, Vol. 285, Issue 28, pp. 21807-16, 2010

Rose, Behm, Drgon, Johnson, Uhl: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010

Perlis, Smoller, Mysore, Sun, Gillis, Purcell, Rietschel, Nöthen, Witt, Maier, Iosifescu, Sullivan, Rush, Fava, Breiter, Macdonald, Gusella: "Prevalence of incompletely penetrant Huntington's disease alleles among individuals with major depressive disorder." in: The American journal of psychiatry, Vol. 167, Issue 5, pp. 574-9, 2010

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN394366) back to top
Supplier Images
ELISA image for anti-Huntingtin antibody (HTT) (AA 81-191) (ABIN394366) Detection limit for recombinant GST tagged HD is approximately 0.3 ng/mL as a capture...