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anti-Human MAP3K8 antibody for Immunofluorescence

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • c-COT
  • Cot
  • COT
  • Cot/Tpl2
  • D17TPL2
  • Est
  • EST
  • ESTF
  • Estf
  • MAP3K8
  • MEKK8
  • Tpl-2
  • TPL2
  • Tpl2
Epitope
Center
29
16
13
9
7
6
4
4
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
93
37
35
5
4
2
1
1
Host
Rabbit
89
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
41
32
22
13
11
7
4
3
2
Supplier
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Available images

Catalog No. ABIN2855464
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3062192 ABIN2855466 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.3062192 ABIN2707193 IC IF IHC WB Rabbit pSer290 Log in to see Polyclonal
0.3062192 ABIN2448630 IF IHC ELISA WB Rabbit IgG Thr290 Log in to see Polyclonal
0.0010883404 ABIN265369 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010883404 ABIN2888594 IF IHC (p) ELISA WB Rabbit IgG AA 256-305 Log in to see Polyclonal
0.0010883404 ABIN3198352 IC IF IHC WB Rabbit Log in to see Polyclonal
0.0010883404 ABIN2972689 ICC IF WB Rabbit Center Log in to see Polyclonal

Similar anti-MAP3K8 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 13 Antibodies 41 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 7 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 23 Antibodies
Western Blotting (WB) 20 Antibodies 50 Antibodies
Dot Blot (DB) 11 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Epitope Center
(29), (16), (13), (9), (7), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(93), (37), (35), (5), (4), (2), (1), (1)
Host Rabbit
(89), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(49), (41), (32), (22), (13), (11), (7), (4), (3), (2)
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling Images
Cross-Reactivity Cow (Bovine), Rat (Rattus)
Cross-Reactivity (Details) Rat (95 %), Bovine (97 %)
Characteristics Rabbit Polyclonal antibody to MAP3K8 (mitogen-activated protein kinase kinase kinase 8)
MAP3K8 antibody [N3C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MAP3K8. The exact sequence is proprietary.
Isotype IgG

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K8 (MAP3K8 Antibody Abstract)
Background This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.

Molecular Weight 53 kDa
Gene ID 1326
Research Area Cancer
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (Center) (ABIN2855464) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using MAP3K8, antibo...
Immunohistochemistry (IHC) image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (Center) (ABIN2855464) IHC-P Image Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , usin...
Immunohistochemistry (IHC) image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (Center) (ABIN2855464) IHC-P Image MAP3K8 antibody [N3C3] detects MAP3K8 protein at cytoplasm on mouse colon...
Western Blotting (WB) image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (Center) (ABIN2855464) WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 10% SDS PAGE antibody dilute...