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anti-Mouse (Murine) MAPKSP1 antibody for Immunoprecipitation

Recommended MAPKSP1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) Antibodies
  • AW556229
  • MAP2K1IP1
  • Map2k1ip1
  • MAPBP
  • Mapbp
  • mapksp1
  • Mapksp1
  • MAPKSP1
  • MP1
  • Mp1
  • PRO0633
  • Ragulator3
  • zgc:110207
Epitope
C-Term
8
6
5
3
2
2
2
Reactivity
Human, Mouse (Murine)
45
26
21
Host
Rabbit
39
6
Clonality
Monoclonal
Conjugate
This MAPKSP1 antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
30
14
11
10
7
6
5
1
1
1
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Available images

Catalog No. ABIN189551
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Similar anti-MAPKSP1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 3 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 7 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 8 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) Antibodies
Epitope C-Term
(8), (6), (5), (3), (2), (2), (2)
Reactivity Human, Mouse (Murine)
(45), (26), (21)
Host Rabbit
(39), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPKSP1 antibody is un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(30), (14), (11), (10), (7), (6), (5), (1), (1), (1)
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Product Details anti-MAPKSP1 Antibody

Target Details MAPKSP1 Application Details Handling Images
No Cross-Reactivity Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in the C-terminus of human mitogen-activated protein kinase kinase 1 interacting protein 1 was used as immunogen.
Clone Y130
Isotype IgG

Target Details MAPKSP1

Product Details anti-MAPKSP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K1IP1 (MAPKSP1 Antibody Abstract)
Background The function of MP1 (MEK partner-1) a 14kDa scaffold protein, is to bind specifically toMEK1 and ERK1 and bring them in close proximity in order to facilitate their activation(1). Localized to the late endosome, MP1 also interacts with an adaptor protein p14.While the interaction between MP1-ERK1 can take place in the absence of the adaptorprotein p14, it is strengthened in the presence of p14 (2). Additionally, it has beensuggested that the binding of MP1 to MEK1resides in a proline-rich sequence (residue270-307) of MEK1. Alternate Names: anti-MEK binding partner 1 antibody, anti-MEK partner 1 antibody, anti-Mitogenactivated protein kinase kinase 1 interacting protein 1 antibody, anti-MP1 antibody.
Gene Symbol: LAMTOR3
Gene ID 8649, 56692
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 14kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000
Protocol Protocol specific for MAP2K1IP1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAPKSP1 antibody (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1) (C-Term) (ABIN189551) Western blot analysis on A431 cell lysate using anti-MP1 (C-term) RabMAb (catalog #11...
Immunohistochemistry (IHC) image for anti-MAPKSP1 antibody (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1) (C-Term) (ABIN189551) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-M...