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anti-Human NCF1 antibody for Western Blotting

Recommended NCF1 Antibody (supplied by: Log in to see )

Antigen
Neutrophil Cytosol Factor 1 (NCF1) Antibodies
  • Ncf-1
  • NCF1A
  • NOXO2
  • p47
  • p47phox
  • SH3PXD1A
Epitope
pSer359
56
22
15
15
13
12
11
8
6
6
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
193
74
68
13
10
9
7
6
5
4
2
1
Host
Rabbit
179
34
2
Clonality
Polyclonal
Conjugate
This NCF1 antibody is un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
165
114
100
29
26
23
12
5
4
2
1
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Catalog No. ABIN1531723
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1532163 IHC ELISA WB Rabbit IgG pSer345 Log in to see Polyclonal 1
0.12303223 ABIN184583 ELISA WB Goat C-Term Log in to see Polyclonal 1
0.12303223 ABIN1533187 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1532751 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1532746 IF ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1532752 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN967918 IF WB Mouse IgG1 AA 18-197 Log in to see 1-p47Phox 5
0.12303223 ABIN967919 IF WB Mouse IgG1 AA 18-197 Log in to see 1-p47Phox 5
0.12303223 ABIN652063 IHC (p) WB Rabbit Ig AA 26-52, N-Term Log in to see Polyclonal
0.12303223 ABIN784240 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.12303223 ABIN1531717 ELISA WB Rabbit IgG pSer304 Log in to see Polyclonal
0.12303223 ABIN498263 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.12303223 ABIN1449574 WB Goat Internal Region Log in to see Polyclonal
0.12303223 ABIN2465255 ELISA WB Goat C-Term Log in to see Polyclonal
0.12303223 ABIN2705083 IHC WB Rabbit C-Term, pSer304 Log in to see Polyclonal
0.12303223 ABIN2705084 IHC WB Rabbit C-Term, pSer328 Log in to see Polyclonal
0.12303223 ABIN2705085 IC IF IHC WB Rabbit C-Term, pSer359 Log in to see Polyclonal
0.12303223 ABIN2705086 IC IF IP WB Rabbit C-Term, pSer370 Log in to see Polyclonal
0.12303223 ABIN2705087 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2446704 IHC ELISA WB Rabbit IgG pSer345 Log in to see Polyclonal

Top referenced anti-NCF1 antibody for Western Blotting

Similar anti-NCF1 Antibodies

Application / Reactivity Human
ELISA 99 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 97 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 149 Antibodies

General

Antigen Neutrophil Cytosol Factor 1 (NCF1) Antibodies
Epitope pSer359
(56), (22), (15), (15), (13), (12), (11), (8), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(193), (74), (68), (13), (10), (9), (7), (6), (5), (4), (2), (1)
Host Rabbit
(179), (34), (2)
Clonality
Polyclonal
Conjugate This NCF1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(165), (114), (100), (29), (26), (23), (12), (5), (4), (2), (1)
Pubmed 1 reference available
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Product Details anti-NCF1 Antibody

Target Details NCF1 Application Details Handling References for anti-NCF1 Antibody (ABIN1531723) Images
Specificity P47 phox (Phospho-Ser359) Antibody detects endogenous levels of p47 phox only when phosphorylated at Ser359.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359.
Isotype IgG

Target Details NCF1

Product Details anti-NCF1 Antibody Application Details Handling References for anti-NCF1 Antibody (ABIN1531723) Images back to top
Antigen
Alternative Name p47 Phox (NCF1 Antibody Abstract)
Background Molecular weight of target protein: 44 kDa
Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox
Molecular Weight 44 kDa
Gene ID 653361
OMIM 233700
UniProt P14598
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-NCF1 Antibody Target Details NCF1 Handling References for anti-NCF1 Antibody (ABIN1531723) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.647047, Hs.655201 (NCBI Gene Symbol: NCF1)

Restrictions For Research Use only

Handling

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details References for anti-NCF1 Antibody (ABIN1531723) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-NCF1 Antibody (ABIN1531723)

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling Images back to top
Background publications

Lomax, Leto, Nunoi, Gallin, Malech: "Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease." in: Science (New York, N.Y.), Vol. 245, Issue 4916, pp. 409-12, 1989

Images

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling References for anti-NCF1 Antibody (ABIN1531723) back to top
Supplier Images
Western Blotting (WB) image for anti-NCF1 antibody (Neutrophil Cytosol Factor 1) (pSer359) (ABIN1531723) Western blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h,...
Immunohistochemistry (IHC) image for anti-NCF1 antibody (Neutrophil Cytosol Factor 1) (pSer359) (ABIN1531723) Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 p...
Immunofluorescence (IF) image for anti-NCF1 antibody (Neutrophil Cytosol Factor 1) (pSer359) (ABIN1531723) Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. ...