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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||19 Antibodies||75 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies||64 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||28 Antibodies|
|Western Blotting (WB)||33 Antibodies|
|Flow Cytometry (FACS)||19 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Antigen||Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies|
|Epitope||AA 379-392, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This PDK2 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PDK2 AntibodyTarget Details PDK2 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for[Pyruvate dehydrogenase[lipoamide]] kinase isozyme 2, mitochondrial(PDK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: pyruvate dehydrogenase kinase, isozyme 2
Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PDK2(379-392aa WRHYQTIQEAGDWC), identical to the related rat and mouse sequences.|
Target Details PDK2Product Details anti-PDK2 Antibody Application Details Handling Images back to top
|Alternative Name||PDK2 (PDK2 Antibody Abstract)|
|Background||PDK2(Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96 % identity with that of the rat PDK2 gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.|
|Research Area||Cancer, Metabolism|
|Pathways||PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis|
Application DetailsProduct Details anti-PDK2 Antibody Target Details PDK2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDK2 Antibody Target Details PDK2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling back to top