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|Antigen||3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PDPK1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-PDPK1 AntibodyTarget Details PDPK1 Application Details Handling Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412873|
The antibody recognizes ~60 kDa PDK1 of human, mouse, and rat origins.
A ~33 kDa cleavage fragment can also been recognized.
Reactivity to other species has not been tested.
|Immunogen||Synthetic peptide surrounding amino acid 542 of human PDK1|
Target Details PDPK1Product Details anti-PDPK1 Antibody Application Details Handling Images back to top
|Alternative Name||PDK1 (PDPK1 Antibody Abstract)|
PDK1 (Phosphoinositide-dependent protein kinase 1) plays an important role in cell signaling pathways and is involved in regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.
Synonyms: PDPK-1 , PDPK1 , PDK1 , hPDK1 , MGC35290 , MGC20087 , PkB-like
|Research Area||Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases|
|Pathways||PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-PDPK1 Antibody Target Details PDPK1 Handling Images back to top
|Application Notes||Western blotting (0.5-4 μg/mL), immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (15-25 μg/mL). However, the optimal concentrations should be determined individually. The antibody recognizes ~60 kDa PDK1 of human, mouse, and rat origins. A ~33 kDa cleavage fragment can also been recognized. Reactivity to other species has not been tested.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||The antibody solution should be gently mixed before use.|
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
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