anti-Human PIK3CA antibody for Flow Cytometry

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
AA 1019-1050, C-Term
29
18
16
14
12
9
8
8
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
161
79
55
7
6
2
2
1
1
1
1
1
Host
Rabbit
131
30
21
1
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
8
8
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
119
80
68
36
16
13
10
9
6
5
2
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1882113
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.634827 ABIN1108651 EIA FACS IF WB Mouse IgG1 Log in to see 4F3 2
14.634827 ABIN969555 ICC FACS ELISA WB Mouse IgG1 Log in to see 4F3 3
14.634827 ABIN4345589 CyTOF ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F3
14.634827 ABIN1844691 ELISA FACS IF/ICC WB Mouse IgG1 Log in to see
1 ABIN2178474 FACS PE Rabbit IgG AA 1010-1050 Log in to see Polyclonal 1
1 ABIN1500200 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F3
1 ABIN5534603 FACS IHC (p) WB Rabbit Ig Fraction AA 1019-1050, C-Term Log in to see Polyclonal
1 ABIN4880939 ELISA FACS ICC WB Mouse IgG1 Log in to see 4F3
1 ABIN2881730 FACS IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN2564537 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1840465 FACS IHC (p) WB Rabbit pTyr317 Log in to see Polyclonal
1 ABIN2335686 FACS ELISA WB Mouse IgG1 Log in to see 13B623
1 ABIN5624370 FACS FITC Rabbit IgG1 kappa pTyr199, pTyr458 Log in to see PI3KY458-1A11
1 ABIN5624369 FACS Rabbit IgG1 kappa pTyr199, pTyr458 Log in to see PI3KY458-1A11
1 ABIN2985364 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2793320 FACS IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 1019-1050, C-Term
(29), (18), (16), (14), (12), (9), (8), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(161), (79), (55), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(131), (30), (21), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PIK3CA antibody is un-conjugated
(8), (8), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(119), (80), (68), (36), (16), (13), (10), (9), (6), (5), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN1882113) Images
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1050 amino acids from the C-terminal region of human PI3KCA.
Clone RB01702
Isotype Ig

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA antibody (ABIN1882113) Images back to top
Antigen
Alternative Name PI3KCA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. PI3KCA has been found to be oncogenic and has been implicated in cervical cancers.

Synonyms: PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA
NCBI Accession NP_006209
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA antibody (ABIN1882113) Images back to top
Application Notes Suggested dilutions:
WB: 1:1000,FACS: 1:10-50,IHC (p): 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA antibody (ABIN1882113) Images back to top
Format Liquid
Concentration 1.71 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-PIK3CA antibody (ABIN1882113)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Zhai, Zhao, Ye, Huang, Tian, Wu, Lin, Lin: "Expression of PIK3CA and FOXP1 in gastric and intestinal non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue type." in: Tumour biology, Vol. 32, Issue 5, pp. 913-20, 2011

Tiwari, Choi, Matsuzawa, Pypaert, MacMicking: "Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria." in: Nature immunology, Vol. 10, Issue 8, pp. 907-17, 2009

Singh, Reddy, Goberdhan, Walsh, Dao, Ngai, Chou, O-Charoenrat, Levine, Rao, Stoffel: "p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas." in: Genes & development, Vol. 16, Issue 8, pp. 984-93, 2002

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN1882113) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1019-1050), (C-Term) antibody (ABIN1882113) PIK3CA Antibody (C-term) (ABIN1882113) flow cytometric analysis of Hela cells (right ...
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1019-1050), (C-Term) antibody (ABIN1882113) PIK3CA Antibody (C-term) (ABIN1882113) flow cytometric analysis of Hela cells (right ...
Immunohistochemistry (IHC) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1019-1050), (C-Term) antibody (ABIN1882113) PIK3CA Antibody (C-term) (ABIN1882113) flow cytometric analysis of Hela cells (right ...