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|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||75 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||31 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||89 Antibodies|
|Antigen||Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies|
|Epitope||AA 1019-1050, C-Term Alternatives|
|Conjugate||This PIK3CA antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PIK3CA AntibodyTarget Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN1882113) Images
|Predicted Reactivity||Cow (Bovine)|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1050 amino acids from the C-terminal region of human PI3KCA.|
Target Details PIK3CAProduct Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN1882113) Images back to top
|Alternative Name||PI3KCA (PIK3CA Antibody Abstract)|
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. PI3KCA has been found to be oncogenic and has been implicated in cervical cancers.
Synonyms: PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN1882113) Images back to top
WB: 1:1000,FACS: 1:10-50,IHC (p): 1:50-100
Optimal working dilution should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN1882113) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.|
|Expiry Date||6 months|
References for anti-PIK3CA Antibody (ABIN1882113)Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
|Product cited in:||
Zhai, Zhao, Ye et al.: "Expression of PIK3CA and FOXP1 in gastric and intestinal non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue type." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 32, Issue 5, pp. 913-20, 2011 (PubMed).
Tiwari, Choi, Matsuzawa et al.: "Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria." in: Nature immunology, Vol. 10, Issue 8, pp. 907-17, 2009 (PubMed).
Singh, Reddy, Goberdhan et al.: "p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas." in: Genes & development, Vol. 16, Issue 8, pp. 984-93, 2002 (PubMed).
ImagesProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN1882113) back to top