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anti-Human PIK3CA antibody for Flow Cytometry

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • 6330412C24Rik
  • C-P3K
  • caPI3K
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • p110
  • p110-alpha
  • p110alpha
  • pi3-k
  • PI3-kinase
  • PI3K
  • pi3k
  • PI3K-alpha
Epitope
AA 1019-1050, C-Term
34
16
14
14
13
9
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
141
48
42
7
4
2
1
1
1
Host
Rabbit
99
23
22
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
87
74
67
28
11
10
8
5
5
3
2
2
1
1
Supplier
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Catalog No. ABIN1882113
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN1108651 EIA FACS IF WB Mouse IgG1 Log in to see 4F3 2
0.3068567 ABIN969555 ICC FACS ELISA WB Mouse IgG1 Log in to see 4F3 3
0.3068567 ABIN2492755 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.3068567 ABIN1844691 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.0010666476 ABIN1500200 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 4F3
0.0010666476 ABIN1840465 FACS IHC (p) WB Rabbit Tyr317 Log in to see Polyclonal
0.0010666476 ABIN2335686 FACS ELISA WB Mouse IgG1 Log in to see 13B623
0.0010666476 ABIN2564537 FACS IHC WB Rabbit IgG Log in to see Polyclonal
0.0010666476 ABIN2985364 FACS IHC WB Rabbit IgG Log in to see Polyclonal
0.0010666476 ABIN2793320 FACS IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for Flow Cytometry

Similar anti-PIK3CA Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
ELISA 68 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 86 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 1019-1050, C-Term
(34), (16), (14), (14), (13), (9), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(141), (48), (42), (7), (4), (2), (1), (1), (1)
Host Rabbit
(99), (23), (22)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PIK3CA antibody is un-conjugated
(6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(87), (74), (67), (28), (11), (10), (8), (5), (5), (3), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN1882113) Images
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1050 amino acids from the C-terminal region of human PI3KCA.
Clone RB01702
Isotype Ig

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN1882113) Images back to top
Antigen
Alternative Name PI3KCA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. PI3KCA has been found to be oncogenic and has been implicated in cervical cancers.

Synonyms: PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA
NCBI Accession NP_006209
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN1882113) Images back to top
Application Notes Suggested dilutions:
WB: 1:1000,FACS: 1:10-50,IHC (p): 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN1882113) Images back to top
Format Liquid
Concentration 1.71 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-PIK3CA Antibody (ABIN1882113)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Zhai, Zhao, Ye, Huang, Tian, Wu, Lin, Lin: "Expression of PIK3CA and FOXP1 in gastric and intestinal non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue type." in: Tumour biology, Vol. 32, Issue 5, pp. 913-20, 2011

Tiwari, Choi, Matsuzawa, Pypaert, MacMicking: "Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria." in: Nature immunology, Vol. 10, Issue 8, pp. 907-17, 2009

Singh, Reddy, Goberdhan, Walsh, Dao, Ngai, Chou, O-Charoenrat, Levine, Rao, Stoffel: "p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas." in: Genes & development, Vol. 16, Issue 8, pp. 984-93, 2002

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN1882113) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1019-1050) (ABIN1882113) PIK3CA Antibody (C-term) (ABIN1882113) flow cytometric analysis of Hela cells (right ...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1019-1050) (ABIN1882113) PIK3CA Antibody (C-term) (ABIN1882113) flow cytometric analysis of Hela cells (right ...
Immunohistochemistry (IHC) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1019-1050) (ABIN1882113) PIK3CA Antibody (C-term) (ABIN1882113) flow cytometric analysis of Hela cells (right ...