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anti-Human PIK3CA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
AA 1010-1050
32
16
15
14
13
9
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
61
55
8
6
2
2
2
1
1
1
1
Host
Rabbit
106
26
23
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
82
70
32
27
12
10
7
6
5
2
2
1
1
1
1
Options
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Catalog No. ABIN677198
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.06848 ABIN4345590 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 2
13.06848 ABIN337177 IHC (p) ELISA Goat AA 878-891 Log in to see Polyclonal
13.06848 ABIN498480 IHC (p) WB Rabbit Log in to see Polyclonal
13.06848 ABIN4345591 ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN392560 IHC (p) WB Rabbit Ig AA 504-533, Center Log in to see Polyclonal 3
1 ABIN1882113 FACS IHC (p) WB Rabbit Ig AA 1019-1050, C-Term Log in to see Polyclonal 3
1 ABIN375147 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 1
1 ABIN4620510 EIA IHC (p) WB Rabbit Ig Middle Region Log in to see Polyclonal 4
1 ABIN372794 EIA IHC (p) Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1449335 IHC (p) Rabbit IgG Internal Region Log in to see SP139
1 ABIN1687743 IHC (p) Rabbit IgG Internal Region Log in to see SP139
1 ABIN1687660 IHC (p) Rabbit IgG Internal Region Log in to see SP139
1 ABIN2854758 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN626004 IHC (p) IP ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1738100 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal
1 ABIN1804114 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN2626807 IHC (p) Mouse IgG2a AA 303-631 Log in to see 6D1
1 ABIN2626808 IHC (p) Mouse IgG2a AA 303-631 Log in to see 8D6
1 ABIN2626809 IHC (p) Mouse IgG2a AA 303-631 Log in to see 7A7
1 ABIN2626810 IHC (p) Mouse IgG2a AA 303-631 Log in to see 6D9

Top referenced anti-PIK3CA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PIK3CA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 9 Antibodies 11 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 17 Antibodies 17 Antibodies
Immunofluorescence (IF) 2 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 20 Antibodies 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 16 Antibodies
Immunoprecipitation (IP) 8 Antibodies 7 Antibodies
Western Blotting (WB) 35 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 1010-1050
(32), (16), (15), (14), (13), (9), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(153), (61), (55), (8), (6), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(106), (26), (23)
Clonality
Polyclonal
Conjugate This PIK3CA antibody is un-conjugated
(6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(96), (82), (70), (32), (27), (12), (10), (7), (6), (5), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN677198) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human PI3KCA
Isotype IgG

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN677198) Images back to top
Antigen
Alternative Name Pi3kca (PIK3CA Antibody Abstract)
Background

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.

Subcellular location: Cytoplasm, Membrane bound


Synonyms: MCM, CWS5, MCAP, PI3K, CLOVE, MCMTC, p11-alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha, PtdIns-3-kinase subunit p11-alpha, p11alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PIK3CA
Gene ID 5290
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN677198) Images back to top
Application Notes WB(1:100-1000), FCM(1:20-100), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN677198) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PIK3CA Antibody (ABIN677198)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Paul-Samojedny, Suchanek, Borkowska, Pude?ko, Owczarek, Kowalczyk, Machnik, Fila-Dani?ow, Kowalski: "Knockdown of AKT3 (PKB?) and PI3KCA suppresses cell viability and proliferation and induces the apoptosis of glioblastoma multiforme T98G cells." in: BioMed research international, Vol. 2014, pp. 768181, 2014

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN677198) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1010-1050) (ABIN677198) Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1010-1050) (ABIN677198) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boil...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1010-1050) (ABIN677198) HeLa Cell lysates probed with PI3KCA Polyclonal Antibody, unconjugated at 1:300 over...