B-Cell Linker Protein (BLNK) (AA 1-456) (His tag)

Catalog No. ABIN1096137

Product Details

Target: B-Cell Linker (BLNK)

Protein Type: Recombinant

Protein Characteristics: AA 1-456

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This B-Cell Linker protein is labelled with His tag.

Purpose: Recombinant Human B-Cell Linker Protein/BLNK (C-6His)

Sequence: MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVSLEHH HHHH

Characteristics: Recombinant Human B-Cell Linker Protein/BLNK is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser456) of Human BLNK with a 6His tag at the C-Terminal.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: B-Cell Linker (BLNK)

Alternative Name: B-Cell Linker Protein/BLNK (BLNK Products)

Synonyms: BLNK Protein, blnk Protein, MGC147045 Protein, BASH Protein, Bca Protein, Ly-57 Protein, Ly57 Protein, Lyw-57 Protein, SLP-65 Protein, AGM4 Protein, BLNK-S Protein, LY57 Protein, SLP65 Protein, bca Protein, B-cell linker Protein, B cell linker Protein, BLNK Protein, blnk Protein, Blnk Protein

Sub Type: Fusionprotein

Background: B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.
Alternative Names: B-Cell Linker Protein, B-Cell Adapter Containing a SH2 Domain Protein, B-Cell Adapter Containing a Src Homology 2 Domain Protein, Cytoplasmic Adapter Protein, Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa, SLP-65, BLNK, BASH, SLP65

Molecular Weight: 51.5 kDa

UniProt: Q8WV28

Pathways: BCR Signaling