EDF1 Protein (AA 2-148) (His tag)
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- Target See all EDF1 Proteins
- EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-148
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EDF1 protein is labelled with His tag.
- Purpose
- Recombinant Human Endothelial Differentiation-Related Factor 1/EDF1/MBF1 (C-6His)
- Sequence
- AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ HSITKNTAKL DRETEELHHD RVTLEVGKVI QQGRQSKGLT QKDLATKINE KPQVIADYES GRAIPNNQVL GKIERAIGLK LRGKDIGKPI EKGPRAKLEH HHHHH
- Characteristics
- Recombinant Human Endothelial Differentiation-Related Factor 1/EDF1 is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Lys148) of Human EDF1 fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product EDF1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
- Alternative Name
- EDF1 (EDF1 Products)
- Synonyms
- edf-1 Protein, mbf1 Protein, EDF-1 Protein, MBF1 Protein, 0610008L11Rik Protein, AA409425 Protein, CAP-19 Protein, zgc:73192 Protein, zgc:86829 Protein, endothelial differentiation related factor 1 S homeolog Protein, endothelial differentiation related factor 1 Protein, endothelial differentiation-related factor 1 Protein, Mbf1p Protein, edf1.S Protein, EDF1 Protein, Edf1 Protein, edf1 Protein, MBF1 Protein
- Sub Type
- Fusionprotein
- Background
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Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.
Alternative Names: Endothelial Differentiation-Related Factor 1, EDF-1, Multiprotein-Bridging Factor 1, MBF1, EDF1 - Molecular Weight
- 17.4 kDa
- UniProt
- O60869
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