EDF1 Protein (His tag)

Catalog No. ABIN7318429

Product Details

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EDF1 protein is labelled with His tag.

Purpose: Recombinant Human EDF1/MBF1 Protein (His Tag)

Sequence: Ala2-Lys148

Characteristics: Recombinant Human Endothelial Differentiation-Related Factor 1 is produced by our E.coli expression system and the target gene encoding Ala2-Lys148 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1/MBF1 (EDF1 Products)

Synonyms: edf-1 Protein, mbf1 Protein, EDF-1 Protein, MBF1 Protein, 0610008L11Rik Protein, AA409425 Protein, CAP-19 Protein, zgc:73192 Protein, zgc:86829 Protein, endothelial differentiation related factor 1 S homeolog Protein, endothelial differentiation related factor 1 Protein, endothelial differentiation-related factor 1 Protein, Mbf1p Protein, edf1.S Protein, EDF1 Protein, Edf1 Protein, edf1 Protein, MBF1 Protein

Background:

Background: Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.

Synonym: Endothelial Differentiation-Related Factor 1, EDF-1, Multiprotein-Bridging Factor 1, MBF1, EDF1

Molecular Weight: 17.4 kDa

UniProt: O60869