POMGNT1 Protein (AA 1-660) (His tag)
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- Target See all POMGNT1 Proteins
- POMGNT1 (Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-660
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This POMGNT1 protein is labelled with His tag.
- Purpose
- Recombinant Human POMGNT1 (C-6His)
- Sequence
- LDTRRAISEA NEDPEPEQDY DEALGRLEPP RRRGSGPRRV LDVEVYSSRS KVYVAVDGTT VLEDEAREQG RGIHVIVLNQ ATGHVMAKRV FDTYSPHEDE AMVLFLNMVA PGRVLICTVK DEGSFHLKDT AKALLRSLGS QAGPALGWRD TWAFVGRKGG PVFGEKHSKS PALSSWGDPV LLKTDVPLSS AVEAECHWAD TELNRRRRRF CSKVEGYGSV CSCKDPTPIE FSPDPLPDNK VLNVPVAVIA GNRPNYLYRM LRSLLSAQGV SPQMITVFID GYYEEPMDVV ALFGLRGIQH TPISIKNARV SQHYKASLTA TFNLFPEAKF AVVLEEDLDI AVDFFSFLSQ SIHLLEEDDS LYCISAWNDQ GYEHTAEDPA LLYRVETMPG LGWVLRRSLY KEELEPKWPT PEKLWDWDMW MRMPEQRRGR ECIIPDVSRS YHFGIVGLNM NGYFHEAYFK KHKFNTVPGV QLRNVDSLKK EAYEVEVHRL LSEAEVLDHS KNPCEDSFLP DTEGHTYVAF IRMEKDDDFT TWTQLAKCLH IWDLDVRGNH RGLWRLFRKK NHFLVVGVPA SPYSVKKPPS VTPIFLEPPP KEEGAPGAPE QTVDHHHHHH
- Characteristics
- Recombinant Human Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1/POMGNT1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Thr660) of Human POMGNT1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product POMGNT1 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- POMGNT1 (Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1))
- Alternative Name
- POMGNT1 (POMGNT1 Products)
- Synonyms
- POMGNT1 Protein, im:7151461 Protein, zgc:158130 Protein, 0610016I07Rik Protein, 4930467B06Rik Protein, GNTI.2 Protein, GnT I.2 Protein, LGMD2O Protein, MDDGA3 Protein, MDDGB3 Protein, MDDGC3 Protein, MEB Protein, MGAT1.2 Protein, gnT-I.2 Protein, protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) Protein, protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) S homeolog Protein, protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase Protein, POMGNT1 Protein, pomgnt1.S Protein, pomgnt1 Protein, Pomgnt1 Protein
- Sub Type
- Fusionprotein
- Background
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Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).
Alternative Names: Protein O-Linked-Mannose Beta-1,2-N-Acetylglucosaminyltransferase 1, POMGnT1, UDP-GlcNAc:Alpha-D-Mannoside Beta-1,2-N-Acetylglucosaminyltransferase I.2, GnT I.2, POMGNT1, MGAT1.2 - Molecular Weight
- 69.3 kDa
- UniProt
- Q8WZA1
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