RGS1 Protein (His tag)
-
- Target See all RGS1 Proteins
- RGS1 (Regulator of G-Protein Signaling 1 (RGS1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RGS1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product RGS1 Protein
-
-
- Comment
-
Synonyms: Regulator of G-protein signaling 1, 1R20, BL34, IER1, IR20
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
-
- Target
- RGS1 (Regulator of G-Protein Signaling 1 (RGS1))
- Alternative Name
- RGS1 (RGS1 Products)
- Synonyms
- 1R20 Protein, BL34 Protein, IER1 Protein, IR20 Protein, RGS1 Protein, 1r20 Protein, bl34 Protein, ier1 Protein, ir20 Protein, regulator of G protein signaling 1 Protein, regulator of G-protein signaling 1 Protein, regulator of G-protein signaling 1 S homeolog Protein, Regulator of G-protein signaling 1 Protein, RGS1 Protein, Rgs1 Protein, rgs1.S Protein, rgs1 Protein
- Background
- RGS1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Recombinant human RGS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 26 kDa (233aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002913
- Pathways
- MAPK Signaling, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events
-