MAPKAP Kinase 3 Protein (His tag)
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- Target See all MAPKAP Kinase 3 (MAPKAPK3) Proteins
- MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MAPKAP Kinase 3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product MAPKAPK3 Protein
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- Comment
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Synonyms: MAP kinase-activated protein kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 20% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))
- Alternative Name
- MAPKAPK3 (MAPKAPK3 Products)
- Synonyms
- 3PK Protein, MAPKAP-K3 Protein, MAPKAP3 Protein, MAPKAPK-3 Protein, MK-3 Protein, AI874665 Protein, MK3 Protein, MapkKapk3 Protein, MAPKAPK3 Protein, mapkapk3 Protein, MGC154360 Protein, 3pk Protein, mapkap3 Protein, zgc:158342 Protein, mitogen-activated protein kinase-activated protein kinase 3 Protein, mitogen-activated protein kinase-activated protein kinase 3 S homeolog Protein, MAPKAPK3 Protein, Mapkapk3 Protein, mapkapk3.S Protein, mapkapk3 Protein
- Background
- MAP kinase-activated protein kinase 3, also known as MAPKAPK3, is involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Recombinant human MAPKAPK3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 45.4 kDa (405aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_004626
- Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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