Streptavidin Protein (HRP)

Catalog No. ABIN1305062

Product Details

Target: Streptavidin

Origin: Streptomyces avidinii

Host: Please inquire

Purification tag / Conjugate: This Streptavidin protein is labelled with HRP.

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Brand: BD Pharmingen™

Application Details

Application Notes: Immunohistochemistry: SAv-HRP is ready to use in its pre-diluted format. Remove the solution from 4°C and let it equilibrate to room temperature before using. The SAv-HRP should be used after the slides are incubated with biotinylated primary or secondary antibody and the unbound antibody is washed away. Apply enough SAv-HRP to cover the tissue sections and incubate 30 minutes at room temperature. Wash 3X with PBS and apply the appropriate substrate for horseradish peroxidase (inquire with technical service for available substrates from BD Pharmingen™).
Note: It is important not to use Azide in any of the buffers when using horseradish peroxidase as azide inactivates the enzyme.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Target Details

Target: Streptavidin

Abstract: Streptavidin Products

Background: Streptavidin-Horseradish peroxidase (SAv-HRP) is a useful detection enzyme for labeling biotinylated-primary or seconday antibodies for detection by immunohistochemistry. The streptavidin has very high affinity for biotin conjugated to the antibody and the horseradish peroxidase serves as the enzyme to change color in different substrates. This pre-diluted SAv-HRP is convenient to use and provides optimal signal amplification and detection. This product is routinely tested by immunohistochemistry.