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LIF Protein (AA 23-202)

LIF Origin: Human, Mouse Host: Escherichia coli (E. coli) Recombinant >95 % (SDS-PAGE) SDS Active
Catalog No. ABIN1344371
  • Target See all LIF Proteins
    LIF (Leukemia Inhibitory Factor (LIF))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 23-202
    Origin
    • 22
    • 15
    • 4
    • 3
    • 2
    • 2
    • 1
    Human, Mouse
    Source
    • 21
    • 16
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Specificity
    Mouse and human embryonic stem cells.
    Cross-Reactivity
    Human, Mouse (Murine)
    Characteristics
    Human LIF (aa 23-202) is fused at the N-terminus to a tag.
    Purity
    >95 % (SDS-PAGE)
    Endotoxin Level
    <0.05EU/μg purified protein (LAL test, Lonza).
    Top Product
    Discover our top product LIF Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    Lot specific
    Buffer
    Lyophilized. Contains PBS.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 6 months after receipt when stored at -20°C.
    Expiry Date
    6 months
  • Target
    LIF (Leukemia Inhibitory Factor (LIF))
    Alternative Name
    LIF (LIF Products)
    Synonyms
    CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein
    Background
    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
    Molecular Weight
    ~46kDa (SDS-PAGE)
    UniProt
    P15018
    Pathways
    JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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