ST8SIA1 Protein (AA 49-356) (His tag)
-
- Target See all ST8SIA1 Proteins
- ST8SIA1 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 1 (ST8SIA1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 49-356
-
Origin
- Human
-
Source
- Human Cells
- Purification tag / Conjugate
- This ST8SIA1 protein is labelled with His tag.
- Purpose
- Recombinant Human α-N-Acetylneuraminide α-2,8-Sialyltransferase/ST8SIA1 (C-6His)
- Sequence
- YRLPNEKEIV QGVLQQGTAW RRNQTAARAF RKQMEDCCDP AHLFAMTKMN SPMGKSMWYD GEFLYSFTID NSTYSLFPQA TPFQLPLKKC AVVGNGGILK KSGCGRQIDE ANFVMRCNLP PLSSEYTKDV GSKSQLVTAN PSIIRQRFQN LLWSRKTFVD NMKIYNHSYI YMPAFSMKTG TEPSLRVYYT LSDVGANQTV LFANPNFLRS IGKFWKSRGI HAKRLSTGLF LVSAALGLCE EVAIYGFWPF SVNMHEQPIS HHYYDNVLPF SGFHAMPEEF LQLWYLHKIG ALRMQLDPCE DTSLQPTSVD HHHHHH
- Characteristics
- Recombinant Human alpha-N-Acetylneuraminide alpha-2,8-Sialyltransferase/ST8SIA1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Tyr49-Ser356) of Human ST8SIA1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ST8SIA1 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
-
- Target
- ST8SIA1 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 1 (ST8SIA1))
- Alternative Name
- alpha-2-8-Sialyltransferase-8A (ST8SIA1 Products)
- Synonyms
- SIAL-T2 Protein, Xlgd3 Protein, siat8 Protein, siat8a Protein, st8sial Protein, st8siaI Protein, ST8SIA1 Protein, St8sia1 Protein, GD3S Protein, SIAT8 Protein, SIAT8-A Protein, SIAT8A Protein, ST8SiaI Protein, 9330109E03Rik Protein, Sia-T Protein, Siat8 Protein, Siat8a Protein, ST8SIA-IV Protein, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 Protein, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 L homeolog Protein, ST8SIA1 Protein, st8sia1.L Protein, st8sia1 Protein, St8sia1 Protein
- Sub Type
- Fusionprotein
- Background
-
Alpha-N-Acetylneuraminide alpha-2,8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus.
Alternative Names: Alpha-N-Acetylneuraminide Alpha-2,8-Sialyltransferase, Alpha-2,8-Sialyltransferase 8A, Ganglioside GD3 Synthase, Ganglioside GT3 Synthase, Sialyltransferase 8A, SIAT8-A, Sialytransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A - Molecular Weight
- 36.2 kDa
- UniProt
- Q92185
-