GLN1 Protein (AA 2-373) (His tag)
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- Target See all GLN1 Proteins
- GLN1 (Glutamine Synthetase (GLN1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-373
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GLN1 protein is labelled with His tag.
- Purpose
- Recombinant Human Glutamine Synthetase/GLUL (C-6His)
- Sequence
- TTSASSHLNK GIKQVYMSLP QGEKVQAMYI WIDGTGEGLR CKTRTLDSEP KCVEELPEWN FDGSSTLQSE GSNSDMYLVP AAMFRDPFRK DPNKLVLCEV FKYNRRPAET NLRHTCKRIM DMVSNQHPWF GMEQEYTLMG TDGHPFGWPS NGFPGPQGPY YCGVGADRAY GRDIVEAHYR ACLYAGVKIA GTNAEVMPAQ WEFQIGPCEG ISMGDHLWVA RFILHRVCED FGVIATFDPK PIPGNWNGAG CHTNFSTKAM REENGLKYIE EAIEKLSKRH QYHIRAYDPK GGLDNARRLT GFHETSNIND FSAGVANRSA SIRIPRTVGQ EKKGYFEDRR PSANCDPFSV TEALIRTCLL NETGDEPFQY KNLEHHHHHH
- Characteristics
- Recombinant Human Glutamine Synthetase/GLUL is produced by our E. coli expression system. The target protein is expressed with sequence (Thr2-Asn373) of Human GLUL fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 50 mM Imidazole, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- GLN1 (Glutamine Synthetase (GLN1))
- Alternative Name
- Glutamine Synthetase (GLN1 Products)
- Synonyms
- GLNS Protein, GS Protein, PIG43 Protein, PIG59 Protein, Glns Protein, glutamate-ammonia ligase Protein, glutamate-ammonia ligase (glutamine synthetase) Protein, glutamine synthetase Protein, GLUL Protein, Glul Protein, GLN1 Protein
- Sub Type
- Fusionprotein
- Background
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Glutamine Synthetase reglutes intracellular concentration of glutamate. Glutamine Synthetase catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is an important source of energy and that takes part in cell prolifetation, inhibition of apoptosis, and cell signaling. Glutamine Synthetase is expressed during early fetal stages, and has a role in maintaining body PH by removing ammonia from circulation. Mutations in the GLUL gene are related to congenital glutamine deficiency.
Alternative Names: Glutamine Synthetase, GS, Glutamate Decarboxylase, Glutamate--Ammonia Ligase, GLUL, GLNS - Molecular Weight
- 43.13 kDa
- UniProt
- P15104
- Pathways
- Positive Regulation of Peptide Hormone Secretion
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