BDH1 Protein (AA 1-343) (His tag)
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- Target See all BDH1 Proteins
- BDH1 (3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-343
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BDH1 protein is labelled with His tag.
- Purpose
- Recombinant Human D-β-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MLATRLSRPL SRLPGKTLSA CDRENGARRP LLLGSTSFIP IGRRTYASAA EPVGSKAVLV TGCDSGFGFA LAKHLHSKGF LVFAGCLMKD KGHDGVKELD SLNSDRLRTV QLNVCSSEEV EKVVEIVRSS LKDPEKGMWG LVNNAGISTF GEVEFTSLET YKQVAEVNLW GTVRMTKSFL PLIRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMYPLG VKVSVVEPGN FIAATSLYSP ESIQAIAKKM WEELPEVVRK DYGKKYFDEK IAKMETYCSS GSTDTSPVID AVTHALTATT PYTRYHPMDY YWWLRMQIMT HLPGAISDMI YIR
- Characteristics
- Recombinant Human D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg343) of Human BDH1 fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BDH1 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM Tris, 1 mM DTT, pH 7.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- BDH1 (3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))
- Alternative Name
- D-beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 (BDH1 Products)
- Synonyms
- zgc:163053 Protein, BDH Protein, SDR9C1 Protein, 2310032J20Rik Protein, AI327223 Protein, Bdh Protein, 3-hydroxybutyrate dehydrogenase 1 Protein, 3-hydroxybutyrate dehydrogenase 1 S homeolog Protein, 3-hydroxybutyrate dehydrogenase, type 1 Protein, 3-hydroxybutyrate dehydrogenase, type 1 A Protein, BDH1 Protein, bdh1.S Protein, bdh1 Protein, Bdh1 Protein, BDH1A Protein
- Sub Type
- Fusionprotein
- Background
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D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.
Alternative Names: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial, BDH, 3-Hydroxybutyrate Dehydrogenase, BDH - Molecular Weight
- 40.3 kDa
- UniProt
- Q02338
- Pathways
- Response to Growth Hormone Stimulus
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