BPGM Protein (AA 2-259) (His tag)
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- Target See all BPGM Proteins
- BPGM (2,3-bisphosphoglycerate Mutase (BPGM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-259
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BPGM protein is labelled with His tag.
- Purpose
- Recombinant Human Bisphosphoglycerate Mutase/BPGM (C-6His)
- Sequence
- SKYKLIMLRH GEGAWNKENR FCSWVDQKLN SEGMEEARNC GKQLKALNFE FDLVFTSVLN RSIHTAWLIL EELGQEWVPV ESSWRLNERH YGALIGLNRE QMALNHGEEQ VRLWRRSYNV TPPPIEESHP YYQEIYNDRR YKVCDVPLDQ LPRSESLKDV LERLLPYWNE RIAPEVLRGK TILISAHGNS SRALLKHLEG ISDEDIINIT LPTGVPILLE LDENLRAVGP HQFLGDQEAI QAAIKKVEDQ GKVKQAKKLE HHHHHH
- Characteristics
- Recombinant Human Bisphosphoglycerate Mutase/BPGM is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys259) of Human BPGM fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BPGM Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 1 mM DTT, pH 8.0 .
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- BPGM (2,3-bisphosphoglycerate Mutase (BPGM))
- Alternative Name
- Bisphosphoglycerate Mutase/BPGM (BPGM Products)
- Synonyms
- DPGM Protein, AI323730 Protein, AL022789 Protein, C86192 Protein, Ab2-098 Protein, zgc:92230 Protein, Bisphosphoglycerate mutase Protein, bisphosphoglycerate mutase Protein, 2,3-bisphosphoglycerate mutase Protein, bisphosphoglycerate mutase S homeolog Protein, pmge Protein, BPGM Protein, Bpgm Protein, bpgm Protein, bpgm.S Protein
- Sub Type
- Fusionprotein
- Background
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Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily. BPGM is a multifunctional enzyme. BPGM catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. It also has phosphoglycerate phosphomutase activity. BPGM plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Deficiency of BPGM increases the affinity of cells for oxygen and result in hemolytic anemia.
Alternative Names: Bisphosphoglycerate Mutase, BPGM, 2,3-Bisphosphoglycerate Mutase Erythrocyte, 2,3-Bisphosphoglycerate Synthase, 2,3-Diphosphoglycerate Mutase, DPGM, BPG-Dependent PGAM, BPGM - Molecular Weight
- 31 kDa
- UniProt
- P07738
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