BPGM Protein (AA 1-259) (His tag)
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- Target See all BPGM Proteins
- BPGM (2,3-bisphosphoglycerate Mutase (BPGM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-259
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BPGM protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- BPGM, 1-259aa, Human, His-tagged, Recombinant, E.coli
- Purity
- > 95 % by SDS - PAGE
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
- Storage
- 4 °C
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- Target
- BPGM (2,3-bisphosphoglycerate Mutase (BPGM))
- Alternative Name
- BPGM (BPGM Products)
- Synonyms
- DPGM Protein, AI323730 Protein, AL022789 Protein, C86192 Protein, Ab2-098 Protein, zgc:92230 Protein, Bisphosphoglycerate mutase Protein, bisphosphoglycerate mutase Protein, 2,3-bisphosphoglycerate mutase Protein, bisphosphoglycerate mutase S homeolog Protein, pmge Protein, BPGM Protein, Bpgm Protein, bpgm Protein, bpgm.S Protein
- Background
- Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2,3-BPG concentration. It is responsible for the catalytic synthesis of 2,3-Bisphosphoglycerate (2,3-BPG) from 1,3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active. Recombinant human BPGM, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192. NCBI no.: NP_001715
- Molecular Weight
- 31kDa (267aa), confirmed by MALDI-TOF
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