Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 22-401) (Active) protein (Fc Tag)

Details for Product No. ABIN2181849, Supplier: Log in to see
Protein Name
  • OCIF
  • OPG
  • TR1
  • BTF3L1
  • HUMBTFB
  • TNFRSF11B
  • Opg
  • ocif
  • opg
  • tr1
  • tumor necrosis factor receptor superfamily, member 11b
  • basic transcription factor 3 pseudogene 11
  • tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
  • osteoprotegerin
  • TNFRSF11B
  • BTF3P11
  • Tnfrsf11b
  • LOC100037695
  • tnfrsf11b
Protein Characteristics
AA 22-401
11
2
2
1
1
1
1
1
Origin
Human
33
4
1
1
1
Source
HEK-293 Cells
9
5
4
3
3
3
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
Fc Tag
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Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. As a result of glycosylation, the protein migrates as 66-85 kDa under reducing (R) condition, and 130-170 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 16 - 32 ng/mL in the presence of 10 ng/mL recombinant human TRAIL.
Background Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Molecular Weight 70.2 kDa
NCBI Accession NP_002537
UniProt O00300
Research Area Cardiovascular, Atherosclerosis, Extracellular Matrix, Innate Immunity, Cytokines
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 22-401) (Active) protein (Fc Tag) (ABIN2181849) Human Osteoprotegerin, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) con...
Background publications Luan, Lu, Jiang, Zhang, Wang, Yuan, Zhao, Wang, Wang: "Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 1, pp. 245-52, 2012 (PubMed).

Prichett, Patton, Field, Brun, Emery, Tan, Rieman, McClung, Nadeau, Mooney, Suva, Gowen, Nuttall: "Identification and cloning of a human urea transporter HUT11, which is downregulated during adipogenesis of explant cultures of human bone." in: Journal of cellular biochemistry, Vol. 76, Issue 4, pp. 639-50, 2000 (PubMed).

Emery, McDonnell, Burke, Deen, Lyn, Silverman, Dul, Appelbaum, Eichman, DiPrinzio, Dodds, James, Rosenberg, Lee, Young: "Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14363-7, 1998 (PubMed).

Tsuda, Goto, Mochizuki, Yano, Kobayashi, Morinaga, Higashio: "Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis." in: Biochemical and biophysical research communications, Vol. 234, Issue 1, pp. 137-42, 1997 (PubMed).