IL-9 Protein (AA 19-144)
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- Target See all IL-9 (IL9) Proteins
- IL-9 (IL9) (Interleukin 9 (IL9))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 19-144
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Origin
- Mouse
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Source
- Insect Cells
- Application
- Flow Cytometry (FACS)
- Purity
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
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Less than 0.1 EU per μg of protein as determine by the LAL method.
- Top Product
- Discover our top product IL9 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: Mouse IL-9 induces proliferation of MO7e cells. The ED50 is 0.04 - 0.16 ng/ml, corresponding to a specific activity of 6.5 - 25.0 x 106 units/mg.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- For maximum results, quick spin vial prior to opening. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- Buffer
- 0.22 μm filtered protein solution is in PBS.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Target
- IL-9 (IL9) (Interleukin 9 (IL9))
- Alternative Name
- IL-9 (IL9 Products)
- Synonyms
- IL9 Protein, IL-9 Protein, Il-9 Protein, P40 Protein, HP40 Protein, interleukin 9 Protein, IL9 Protein, il9 Protein, Il9 Protein
- Background
- IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play important roles in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. Also, IL-9 has been associated with atopic dermatitis.
- Molecular Weight
- The 125 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa. The DTT-reduced protein migrate as multiple bands between 17-25 kDa by SDS-PAGE due to different glycosylation. The predicted N-terminal amino acid is Gln.
- Pathways
- JAK-STAT Signaling
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