Interleukin 9 (IL9) (AA 19-144) (Active) Protein

Details for Product No. ABIN2666759, Supplier: Log in to see
Protein Name
  • IL9
  • IL-9
  • Il-9
  • P40
  • HP40
  • interleukin 9
  • IL9
  • il9
  • Il9
Protein Characteristics
AA 19-144
12
3
2
1
Origin
Human
40
18
4
Source
Insect Cells
39
9
3
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Flow Cytometry (FACS)
Options
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Purity > 90 % , as determined by Coomassie stained SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level

Less than 0.1 EU per μg of protein as determine by the LAL method.

Background IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα ) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.
Molecular Weight The 126 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa. The protein migrates as multiple bands with the molecular mass of 18 - 25 kDa band in SDS-PAGE in DTT-reducing conditions due to different glycosylation. The N-
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Biological activity: Human IL-9 induces proliferation of MO7e cells. ED50=0.1-0.5 ng/mL, corresponding to a specific activity of 2-10 x 106 units/mg.

Restrictions For Research Use only
Format Liquid
Reconstitution For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
Buffer 0.22 μm filtered protein solution is in PBS.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage -20 °C
Storage Comment Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
Supplier Images
ELISA image for Interleukin 9 (IL9) (AA 19-144) (Active) protein (ABIN2666759) Interleukin 9 (IL9) (AA 19-144) (Active) protein
Background publications Sismanopoulos, Delivanis, Alysandratos, Angelidou, Vasiadi, Therianou, Theoharides: "IL-9 induces VEGF secretion from human mast cells and IL-9/IL-9 receptor genes are overexpressed in atopic dermatitis." in: PLoS ONE, Vol. 7, Issue 3, pp. e33271, 2012 (PubMed).

Goswami, Kaplan: "A brief history of IL-9." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 6, pp. 3283-8, 2011 (PubMed).

Tan, Aziz, Lovaas, Vistica, Shi, Wawrousek, Gery: "Antigen-specific Th9 cells exhibit uniqueness in their kinetics of cytokine production and short retention at the inflammatory site." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 11, pp. 6795-801, 2010 (PubMed).

Kaiko, Foster: "New insights into the generation of Th2 immunity and potential therapeutic targets for the treatment of asthma." in: Current opinion in allergy and clinical immunology, Vol. 11, Issue 1, pp. 39-45, 2010 (PubMed).

Jäger, Dardalhon, Sobel, Bettelli, Kuchroo: "Th1, Th17, and Th9 effector cells induce experimental autoimmune encephalomyelitis with different pathological phenotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7169-77, 2009 (PubMed).

Townsend, Fallon, Matthews, Smith, Jolin, McKenzie: "IL-9-deficient mice establish fundamental roles for IL-9 in pulmonary mastocytosis and goblet cell hyperplasia but not T cell development." in: Immunity, Vol. 13, Issue 4, pp. 573-83, 2000 (PubMed).

Mock, Krall, Kozak, Nesbitt, McBride, Renauld, Van Snick: "IL9 maps to mouse chromosome 13 and human chromosome 5." in: Immunogenetics, Vol. 31, Issue 4, pp. 265-70, 1990 (PubMed).

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