CD33 Protein (CD33) (AA 18-259, C-Term) (Fc Tag)
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- Target See all CD33 Proteins
- CD33
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 18-259, C-Term
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD33 protein is labelled with Fc Tag.
- Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC)
- Purity
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
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Less than 0.1 EU per μg of protein as determined by the LAL method.
- Top Product
- Discover our top product CD33 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: Recombinant human Siglec 3 is able to agglutinate human red blood cells with an ED50 = 0.5 - 4.0 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA. After dilution, the cytokine can be stored at 4 °C for one month or from -20 °C to -70 °C for up to three months.
- Concentration
- 100 μg/mL
- Buffer
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- Target
- CD33
- Alternative Name
- Siglec 3 (CD33 Products)
- Synonyms
- SIGLEC-3 Protein, SIGLEC3 Protein, p67 Protein, Siglec-3 Protein, gp67 Protein, CD33 molecule Protein, CD33 antigen Protein, CD33 Protein, Cd33 Protein
- Background
- Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most Siglecs contain a combination of tyrosine motifs, including immunoreceptor tyrosine-based inhibitory motifs (ITIM), along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that Siglecs may participate in cell-cell interactions and act as receptors for the entry of viral or bacterial pathogens. In addition, the presence of ITIM indicates that these molecules play a role in the suppression of immunoreceptor signaling. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4, as one group and a Siglec 3/CD33-related subgroup (Siglec 3 and 5 through 14) as the second. Siglec 3, also known as CD33 and GP67, is mainly expressed on myeloid cells and also on activated NK and T cells. Human Siglec 3 has two tyrosine residues in its cytoplasmic domain. When phosphorylated, these tyrosines could confer an inhibitory function. CD33 polymorphism has been associated with late-onset Alzheimer's disease.
- Molecular Weight
- This 493 amino acid recombinant protein has a predicted molecular mass of approximately 54.9 kDa. The protein migrates approximately at 70 kDa in DTT-reducing conditions and at 150 kDa in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino
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