Thrombopoietin Protein (THPO) (AA 22-195)

Catalog No. ABIN2667585

Product Details

Target: Thrombopoietin (THPO)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 22-195

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: The expected ED50 is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg, as determined by the dose-dependent stimulation of the proliferation of human MO7e cells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin (THPO Products)

Synonyms: MGDF Protein, MKCSF Protein, ML Protein, MPLLG Protein, THCYT1 Protein, TPO Protein, Mgdf Protein, Ml Protein, Mpllg Protein, Tpo Protein, Tpo1 Protein, Tpo2 Protein, Tpo3 Protein, Tpo4 Protein, tpo Protein, LOC100231762 Protein, thrombopoietin Protein, THPO Protein, Thpo Protein, thpo Protein

Background: Mouse Thrombopoietin (TPO) is a protein consisting of an N-terminus domain with homology to erythropoietin (EPO) and a C-terminus domain with multiple N-linked and O-linked glycosylation sites. TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. It signals through the c-mpl receptor and stimulates the proliferation and maturation of megakaryocytes, hence, TPO plays an important role in regulating the level of circulating platelets in vivo. It has also been shown that TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitors. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Molecular Weight: The 174 amino acid recombinant protein has a predicted molecular mass of 18.7 kDa. The predicted N-terminal amino acid is Ser.

Pathways: JAK-STAT Signaling, Hormone Activity