PRMT5 Protein (DYKDDDDK Tag)
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- Target See all PRMT5 Proteins
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This PRMT5 protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa.
- Top Product
- Discover our top product PRMT5 Protein
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- Application Notes
- Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3R8 and H4R3 methyltransferase. HMT Assay Conditions:50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl H3 (1-21) peptide, 20 ng/μl Recombinant PRMT5 protein at 2 hours at room temperature. Activity was detected by fluorography.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
- Alternative Name
- PRMT5 (PRMT5 Products)
- Synonyms
- HRMT1L5 Protein, IBP72 Protein, JBP1 Protein, SKB1 Protein, SKB1Hs Protein, Jbp1 Protein, Skb1 Protein, si:zc14a17.2 Protein, skb1 Protein, wu:fb95f10 Protein, zgc:110669 Protein, hsl7 Protein, DDBDRAFT_0187422 Protein, DDBDRAFT_0235403 Protein, DDB_0187422 Protein, DDB_0235403 Protein, NV18830 Protein, jbp1 Protein, ibp72 Protein, skb1hs Protein, hrmt1l5 Protein, protein arginine methyltransferase 5 Protein, protein arginine N-methyltransferase 5 Protein, protein arginine methyltransferase 5 L homeolog Protein, putative arginine N-methyltransferase, type II Protein, Skb1 family protein Protein, Protein arginine N-methyltransferase 5 Protein, PRMT5 Protein, Prmt5 Protein, prmt5 Protein, prmt5.L Protein, LOC100115766 Protein, prmt-5 Protein
- Background
- PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
- Molecular Weight
- 74.2 kDa
- Pathways
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
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